文章摘要
龙兴蓝,许涛,邹丽丽,蒋婷,彭涛,孙衢骎.Wnt/β-catenin通路在脉冲强磁场促神经干细胞增殖中的作用初探[J].中国康复,2018,33(4):267-271
Wnt/β-catenin通路在脉冲强磁场促神经干细胞增殖中的作用初探
Role of Wnt/β-catenin pathway in proliferation of neural stem cells induced by high-intensity pulsed electromagnetic fields
  
DOI:
中文关键词: 神经干细胞  脉冲强磁场  增殖  RNAi  wnt/β-catenin通路
英文关键词: Neural stem cells  High-intensity pulsed electromagnetic fields  Proliferation  RNAi  Wnt/β-catenin pathway
基金项目:国家自然科学基金(面上项目)(81271732)
作者单位
龙兴蓝 三峡大学人民医院康复医学科湖北 宜昌 443000 
许涛 华中科技大学同济医学院附属同济医院康复医学科武汉 430030 
邹丽丽 河南省人民医院康复医学科郑州 450000 
蒋婷 武汉大学中南医院康复医学科武汉 430030 
彭涛 华中科技大学脉冲强磁场中心武汉 430030 
孙衢骎 华中科技大学脉冲强磁场中心武汉 430030 
摘要点击次数: 6576
全文下载次数: 4517
中文摘要:
  目的:摸索抑制神经干细胞(NSCs)中β-catenin表达的合适条件,为获得抑制β-catenin表达的NSCs做准备。利用抑制β-catenin表达的NSCs,探索wnt/β-catenin通路在脉冲强磁场促NSCs增殖中的作用。方法:取新生的SD大鼠双侧侧脑室下的NSCs,然后用无血清培养基培养14d。利用RNAi原理构建β-catenin shRNA慢病毒载体及阴性慢病毒载体,并转染NSCs,确定合适的感染复数(MOI)值。将NSCs分为阴性组(阴性病毒转染组)和实验组(β-catenin shRNA病毒转染组),并用4T、0.1Hz、8次的脉冲强磁场干预。在用脉冲强磁场干预后的1d、7d,采用CCK8法检测阴性组和实验组的NSCs增殖情况。结果:β-catenin shRNA序列在MOI=10时,对NSCs中β-catenin的抑制效果最好。阴性病毒组在脉冲强磁场干预后1d、7d 时NSCs增殖最明显(P<0.05),而实验组在干预前后NSCs增殖水平无明显变化。结论:wnt/β-catenin通路在脉冲强磁场促NSCs增殖中起作用。
英文摘要:
  Objective: To investigate the optimal conditions of inhibiting the β-catenin expression in neural stem cells (NSCs) for harvesting NSCs low-expressing β-catenin, and exploring the roles of Wnt/β-catenin pathway in proliferation of NSCs induced by the high-intensity pulsed electromagnetic fields. Methods: (1) NSCs were derived from subventricular zone (SVZ) of newborn SD rats, and then cultured for 14 days using serum-free medium. (2) The RNAi method was used to inhibit β-catenin expression in NSCs, and the optimal MOI for transfection to inhibit β-catenin levels in NSCs was measured. (3) The NSCs were divided into the negative control group and experimental group, and then they were exposed in the high-intensity pulsed electromagnetic fields with stimulus condition of 4T, 0.1Hz, 8 times. (4) At 1st and 7th day after the intervention with the high-intensity pulsed electromagnetic fields, the proliferation of NSCs was tested by the CCK8 assay. Results: (1) When MOI=10, the best inhibitory effects on β-catenin could be obtained in NSCs. (2) At 1st and 7th day after NSCs were exposed to the high-intensity pulsed electromagnetic fields, the proliferation of NSCs in the negative control group was significantly higher than in the experimental group (P<0.05). Conclusion: The Wnt/β-catenin pathway may play a role in proliferation of NSCs induced by the high-intensity pulsed electromagnetic fields.
查看全文   下载PDF阅读器  HTML全文
关闭
本刊微信二维码