| 张艳丽,吐尔逊·沙比尔.额叶p-mTOR异常表达与外伤性癫痫发生的相关研究[J].中国康复,2018,33(2):91-94 |
| 额叶p-mTOR异常表达与外伤性癫痫发生的相关研究 |
| Assessment of the correlation between posttraumatic epilepsy and the abnormal expression of p-mTOR in frontal lobe |
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| DOI： |
| 中文关键词: 外伤性癫痫 mTOR通路 发病机制 |
| 英文关键词: posttraumatic epilepsy mTOR pathway pathogenesis |
| 基金项目:国家自然科学基金（81260180） |
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| 摘要点击次数: 6636 |
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| 中文摘要: |
| 目的：探索外伤性癫痫（Posttraumatic epilepsy，PTE）发生与额叶p-mTOR异常表达的相关性。方法：将78只成年雄性SD大鼠分为正常对照组（A组）6只，生理盐水对照组（B组）和癫痫模型组（C组）各36只，C组右侧额叶注射FeCl2造模。应用免疫组化技术检测造模后1h、24h、1周、2周、4周各组大鼠额叶皮质中p-mTOR（Ser2448）的动态表达。结果：A组额叶p-mTOR(Ser2448)表达的免疫组织化学染色，神经元胞质呈浅棕色，提示弱阳性表达； B组细胞染色程度较A组加深，p-mTOR(Ser2448)表达阳性细胞数增加；C组可见细胞结构紊乱，胞质呈明显的棕黄色，提示该蛋白在细胞质中呈强阳性表达。C组和B组比较，1h表达开始升高（t=-1.435，P=0.182），但差异无统计学意义，1周表达高峰（t=-4.073，P=0.002），2周降低（t=-2.614，P=0.026），4周时再次升高（t=-2.506，P=0.031），差异具有统计学意义(均P<0.05 )。结论：PTE的发病机制可能与mTOR信号通路上p-mTOR（Ser2448）的表达异常增加有关。 |
| 英文摘要: |
| Objective: To explore the correlation between the abnormal expression of p-mTOR in the frontal lobe and posttraumatic epilepsy (PTE). Methods: Adult male SD rats were randomly divided into control group (n=6), saline group (n=36) and epilepsy group (n=36). Models were established by injecting FeCl2 into the right frontal lobe. Immunohistochemistry was used to detect the dynamic expression of p-mTOR (Ser2448) in the frontal cortex of rats at different time points (1 h, 24 h, 1 week, 2 weeks, and 4 weeks after modeling). Results: As compared with the saline group, the expression of p-mTOR (Ser2448) in the frontal cortex of the epilepsy group began to increase after 1 h (t=-1.435, P=0.182), reached its maximum after 1 week (t=-4.073, P=0.002), reduced after 2 weeks (t=-2.614, P=0.026) and rose again after 4 weeks (t=-2.506, P=0.031). The difference was statistically significant. Conclusion: The pathogenesis of PTE may be related to the abnormal increase of the expression of p-mTOR (Ser2448) protein in the mTOR signaling pathway. |
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