| 付佩彩,喻志源,刘淼,唐荣华,王伟,骆翔.溶血磷脂酸对小胶质细胞活化和吞噬功能的影响[J].中国康复,2012,27(3):166-168 |
| 溶血磷脂酸对小胶质细胞活化和吞噬功能的影响 |
| Effect of LPA on activation and endocytosis of microglia |
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| DOI： |
| 中文关键词: 溶血磷脂酸 小胶质细胞 FITC-葡聚糖 流式细胞术 |
| 英文关键词: LPA microglia FITC-dextran FCM |
| 基金项目:国家自然科学基金项目(81000521，81030021) |
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| 中文摘要: |
| 目的：观察溶血磷脂酸(LPA)对小胶质细胞活化及其吞噬功能的影响。方法：小胶质细胞系BV2细胞复苏后传代培养，取对数生长期细胞分为对照组和LPA组，LPA干预30 min后各组加入FITC标记的葡聚糖，在干预后1 h、2 h后收取细胞，用流式细胞仪测定各孔FITC-葡聚糖微粒吞噬率及荧光强度。结果：LPA干预1 h时，吞噬率较对照组明显增加(P<0.05)，2 h时两组细胞吞噬率达到最高值。LPA干预后1 h及2 h时，吞噬荧光强度较对照组明显增加(P<0.01)，而且吞噬率与荧光强度的乘积较对照组亦明显增加(P<0.01)。结论：LPA可提高BV2细胞活化及吞噬功能，提示LPA在小胶质细胞活化及吞噬过程中发挥了重要作用。 |
| 英文摘要: |
| Objective: To observe the effect of lysophosphatidic acid (LPA) on activation and endocytosis of microglia. Methods: BV2 cells in exponential phase were randomly divided into two groups: control group and LPA group. At time points (1 h and 2 h), cells were harvested for later use. FCM was used to detect the phagocytic rate of FITC-dextran and the fluorescence intensity. Results: As compared with control group, the phagocytic rate of FITC-dextran in LPA group was significantly increased at 1 h (P<0.05). At 2 h, the phagocytic rate of FITC-dextran reached the peak in both two groups. At time points (1 h and 2 h), as compared with control group, the fluorescence intensity in LPA group was increased (P<0.01) and the product of phagocytic rate and fluorescence intensity in LPA group was significantly higher than that of control group (P<0.01). Conclusion: LPA can promote activation and endocytosis of microglia in vitro, suggesting the roles of LPA in activation and endocytosis of microglia. |
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