| 张丽,许康,张允建,郭建敏.自拟复方中药对PD多巴胺能神经元保护作用的研究[J].中国康复,2012,27(4):246-248 |
| 自拟复方中药对PD多巴胺能神经元保护作用的研究 |
| Neuroprotective effect of a new formulated traditional Chinese medicine in the MPTP model mice with Parkinson's disease |
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| DOI： |
| 中文关键词: 帕金森氏病 自拟复方中药 一氧化氮 MPTP |
| 英文关键词: Parkinson's disease formulated traditional Chinese medicine Nitric oxide MPTP |
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| 摘要点击次数: 7997 |
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| 中文摘要: |
| 目的:观察一种新型自拟复方中药对1-甲基-4-苯基-1，2，3，6-四氢吡啶（MPTP）所致小鼠帕金森病（PD）的预防作用及其作用机制。方法： C57BL/6J小鼠80只随机分为正常对照组（A组）、中药对照组（B组）、PD模型组（C组）、中药干预组（D组）各20只；给予B、D组中药灌胃，A、C组给予等量生理盐水灌胃，30d后给予C和D组连续5 d腹腔注射MPTP造成PD模型，A和B组注射等量的生理盐水。干预结束后连续5d内检测行为学变化（爬竿试验、游泳试验）、纹状体DA浓度及一氧化氮系统（nNOS和iNOS）含量。结果：造模后第3～5天，D组较C组爬杆实验分值明显增高（P<0.05）；第1～5天，D组较C组游泳实验分值明显增高（P<0.05，0.01）。造模后第5天，D组DA浓度明显高于C组（P<0.05）；D组iNOS mRNA表达量明显低于C组（P<0.05）。结论：中药自拟方对小鼠PD有一定的防治作用，其机制之一可能是通过调节脑内iNOS的表达发挥作用。 |
| 英文摘要: |
| Objective: To investigate the neuroprotective effect of a new formulated traditional Chinese medicine (FTCM) in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse model of Parkinson's disease (PD). Methods: Eighty C57BL/6 mice were randomly divided into 4 groups: group A (normal control group), group B (FTCM control group), group C (PD group), group D (FTCM plus PD group). Mice in group B and group D were given FTCM by a gavage daily for one month, and those in group A and group C were given equivalent saline. Rats in group C and group D were injected with MPTP 30 mg/(kg.day) daily for 5 days, and those in group A and group B received equivalent saline only. In the consecutive 5 days, behavioral changes, DA in the corpus striatum, nNOS and iNOS were observed. Results: At 3rd to 5th day after the operation, the climb rod test score in group D was significantly higher than in group C (P<0.05), and at 1st to 5th day after the operation, the swim test score in group D was significantly higher than in group C (P<0.05，0.01). At 5th day after operation, DA in group D was significantly higher than in group C (P<0.05). iNOS mRNA in group D was significantly lower than in group C (P<0.05). Conclusion: FTCM can prevent PD rats from impairment by MPTP to some extent probably by regulating the expression of iNOS in the brain. |
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