| 吴洪阳,王华,吴绪平.头穴透刺对急性脑缺血大鼠海马齿状回nestin影响的研究[J].中国康复,2013,28(2):87-89 |
| 头穴透刺对急性脑缺血大鼠海马齿状回nestin影响的研究 |
| Effect of scalp-point penetration needling on the expression of nestin in hippocampal dentate gyrus of rats with acute cerebral ischemia |
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| DOI： |
| 中文关键词: 脑缺血 头穴透刺 神经巢蛋白 |
| 英文关键词: cerebral ischemia scalp-point penetration needling nestin |
| 基金项目:湖北省自然科学基金资助项目（2006ABA061） |
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| 中文摘要: |
| 目的：通过观察头穴透刺对急性脑缺血大鼠海马齿状回Nestin表达的变化,探讨头穴透刺抗脑缺血损伤的作用机理。方法：雄性SD大鼠32只，随机分为正常组、脑缺血组、假手术组、头穴透刺组各8只。采用改良线栓法制作大鼠大脑中动脉栓塞局灶性脑缺血模型，头穴透刺组大鼠待清醒后1h，取百会透前顶、率谷透悬厘进行针刺治疗。治疗后对各组大鼠进行神经功能缺损评分，采用免疫组化法检测海马齿状回神经巢蛋白（nestin）的表达。结果：治疗7d后，头穴透刺组大鼠神经缺损评分明显低于较脑缺血组（P＜0.01）；脑缺血组与正常组及假手术组比较，nestin表达明显增加（P<0.01）；与脑缺血组比较，头穴透刺组nestin阳性表达明显增加（P<0.01）。结论：头穴透刺可以使急性脑缺血大鼠受损神经功能明显恢复，能使脑缺血大鼠海马齿状回nestin的表达明显增加，提示头穴透刺有显著抗脑缺血损伤的作用。 |
| 英文摘要: |
| Objective:To investigate the effect of scalp-point penetration needling on the expression of nestin in hippocampal dentate gyrus of rats with acute cerebral ischemia,and the mechanism for scalp-point penetration needling resisting the injury of acute cerebral ischemia.Methods:All 32 SD rats were randomly divided into four groups,with 8 rats in each group:normal group,sham-operated group,cerebral ischemia group and scalp-point penetration needling group.Focal cerebral ischemia model of rats was made by middle cerebral artery occlusion using a thread block technique.The rats in scalp-point penetration needling group were subjected to electro-acupuncture (EA) at Baihui (GV20) through Qianding (GV21) and Shuaigu (GB8) through Xuanli (GB6) with G6805-Ⅱ electro-acupuncture instrument after the rats woke up.After treatment,the neurologic deficit score was recorded and nestin in hippocampal deneate gyrus was detected by using immunohistochemistry.Results:After treatment for 7 days,the neurologic deficit score in scalp-point penetration needling group was obviously higher than in cerebral ischemia group (P<0.01).As compared with sham-operated group and normal control group,the expression of nestin in hippocampal dentate gyrus of scalp-point penetration needling group was increased greatly (P<0.01).As compared with cerebral ischemia group,the expression of nestin in scalp-point penetration needling group was significantly increased (P<0.01).Conclusion:The neurologic impairment of rats with cerebral ischemia was improved obviously and the expression of nestin in hippocampal dentate gyrus increased by scalp-point penetration needling,indicating that scalp-point penetration needling can resist cerebral ischemia injury. |
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