文章摘要
陈志则,祁月红,张雪,姚文龙,张传汉.慢病毒介导的Cdh1-siRNA在大鼠全脑缺血再灌注损伤后的表达及功能[J].中国康复,2013,28(3):163-166
慢病毒介导的Cdh1-siRNA在大鼠全脑缺血再灌注损伤后的表达及功能
Expression and function of lentivirus-mediated Cdh1-siRNA in global brain ischemia-reperfusion damage of rats
  
DOI:
中文关键词: 脑缺血  细胞周期末期促进复合物  慢病毒  RNA干扰  凋亡
英文关键词: cerebral ischemia  anaphase promoting complex  lentivirus  RNA interference  apoptosis
基金项目:国家自然科学基金(30872452)
作者单位
陈志则 华中科技大学同济医学院附属同济医院麻醉科武汉 430030 
祁月红 华中科技大学同济医学院附属同济医院麻醉科武汉 430030 
张雪 华中科技大学同济医学院附属同济医院麻醉科武汉 430030 
姚文龙 华中科技大学同济医学院附属同济医院麻醉科武汉 430030 
张传汉 华中科技大学同济医学院附属同济医院麻醉科武汉 430030 
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中文摘要:
  目的:探讨慢病毒介导RNA干扰Cdh1的表达对全脑缺血再灌注损伤的影响。方法:将150只雄性SD大鼠随机分成生理盐水组(A组)、空慢病毒组(B组)和重组慢病毒组(C组)各50只。分别给予3组大鼠注射生理盐水、空慢病毒和重组慢病毒,注射3d后采用改良4-VO法建立SD大鼠全脑缺血再灌注损伤模型,采用荧光定量PCR检测大鼠海马组织Cdh1 mRNA表达,Western blot检测Cyclin B的变化,TUNEL法检测海马CA1区凋亡细胞指数(AI)。于全脑缺血再灌注术后第7天行Morris水迷宫测试认知功能的变化。结果:C组Cdh1 mRNA表达明显低于A、B组(P<0.05);AI值及Cyclin B表达均明显高于A、B组(P<0.05);水迷宫测试结果显示,术后第9~11天各时间点C组的寻台潜伏期明显长于A、B组(P<0.05)。结论:细胞周期末期分裂促进复合物及其调节亚基Cdh1可能通过Cyclin B堆积介导缺血性神经元的凋亡。
英文摘要:
  Objective:To investigate the expression and function of Cdh1-siRNA in cerebral ischemia-reperfusion injury of rats.Methods:All 150 male Sprague-Dawlcy rats were randomly divided into normal saline group (group A,n=50),lentivirus vector group (group B,n=50) and recombinant lentivirus group (group C,n=50).The rats in 3 groups were injected with normal saline, lentivirus vector and recombinant lentivirus respectively.At the 3rd day after injection,cerebral ischemia-reperfusion injury model of rat was established by modified four-vessel occlusion (4-VO) method.The expression of Cdh1 mRNA and Cyclin B was detected by quantitative real-time PCR and Western blotting.Apoptosis index (AI) was examined by using TUNEL staining method and the behavior was evaluated with Morris water maze test at the 7th day.Results:The expression of Cdh1 mRNA in group C was significantly lower than that in groups A and B (P<0.05),but that of Cyclin B and the levels of AI in group C were significantly higher than those in groups A and B (P<0.05).In addition,Morris water maze tests revealed that the latent period of seeking the platform in group C was longer than that in the other two groups from the 9th to 11th day after surgery.Conclusion:Cyclin B accumulation by APC-Cdh1 may mediate apoptosis of ischemic neurons.
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