| 马集,卢畅,姜茜,殷林波,刘彦娜,刘克敏.外源性磷酸肌酸对游泳力竭小鼠大脑中谷氨酸和钙-ATP酶活力的影响[J].中国康复,2013,28(3):167-170 |
| 外源性磷酸肌酸对游泳力竭小鼠大脑中谷氨酸和钙-ATP酶活力的影响 |
| Effects of exogenous creatine phosphate on glutamic acid and Ca2+ -ATPase activity in brain of mice after exhaustive exercise |
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| DOI： |
| 中文关键词: 运动性疲劳 磷酸肌酸 谷氨酸 钙 ATP酶 |
| 英文关键词: exhaustive exercise creatine phosphate glutamic acid Ca2+ -ATPase |
| 基金项目:大连医科大学大学生科技创新活动基金资助项目（2013022） |
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| 中文摘要: |
| 目的：研究外源性磷酸肌酸(PCr)对游泳力竭小鼠大脑中谷氨酸（Glu）和钙-ATP酶（Ca2+ -ATPase）活力的影响,以进一步揭示PCr的抗疲劳机制。方法:将44只6周龄小鼠分为力竭对照组12只（A 组）、力竭给药组12只（B组）、游泳8min对照组10只（C组）、游泳8min给药组10只（D组），采取小鼠负重游泳的力竭运动模型，每只小鼠负重量为自身体质量的6%。于游泳前30min，B、D组小鼠经腹腔注射磷酸肌酸钠溶液1000mg/kg；A、C组小鼠注射同等比例生理盐水作为安慰剂。记录力竭组小鼠的力竭游泳时间，采用化学比色法检测4组小鼠大脑中Glu含量和Ca2+ -ATPase的活性。结果：经检测，B组小鼠的力竭游泳时间明显长于A组(P<0.05)。小鼠大脑Glu含量检测显示，B组明显低于A组,D组明显低于C组（P<0.05）；Ca2+ -ATPase活力检测显示，B组明显高于A组,D组明显高于C组（P<0.05）。结论：外源性PCr的抗疲劳机制与增强Ca2+ -ATPase活性和间接降低大脑中的Glu含量有关。 |
| 英文摘要: |
| Objective:To observe the effects of exogenous creatine phosphate on glutamic acid level and Ca2+ -ATPase activity in brain of mice after exhaustive exercise and to further reveal the mechanism of exogenous creatine phosphate in allaying tiredness.Methods:All 36 mice,6-week-age,were divided into 4 groups:exhaustive swimming control group (group A);exhaustive swimming with medication group (group B);8-min swimming control group (group C);and 8-min swimming with medication group (group D).The method of mice weight-loading swimming was used to sets up the model of exhaustive exercise,and each mouse loaded weight with 6% of the mass of itself.Thirty min before the experiment,mice in groups B and D were given the intraperitoneal injection with creatine phosphate sodium by the standard of 1000 mg/kg,and the mice in groups A and C were given the same proportionate normal saline as placebo.The exhaustive swimming time was recorded,and glutamic level and Ca2+ -ATPase activity were measured by using biochemical kits.Results:After testing,the exhaustion time in group B was longer than that in group A (P<0.05).The Glu contents in groups B and D were significantly lower than in groups A and C (P<0.05).Ca2+ -ATPase activity in groups B and group D was significantly higher than that in groups A and C (P<0.05).Conclusion:The mechanism of exogenous creatine phosphate in allaying tiredness may be closely related with increased Ca2+ -ATPase activity and reduced glutamic level. |
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