| 沈夏锋,吴军发,于惠贤,张宇玲,田闪,路微波,胡永善,吴毅.一种适用于康复研究的局灶性颅脑外伤大鼠模型[J].中国康复,2014,29(3):163-166 |
| 一种适用于康复研究的局灶性颅脑外伤大鼠模型 |
| A focal brain trauma model for rehabilitation study |
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| DOI： |
| 中文关键词: 颅脑损伤 控制性皮层损伤模型 动物模型 康复 |
| 英文关键词: traumatic brain injury controlled cortical impact animal model rehabilitation |
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| 摘要点击次数: 9145 |
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| 中文摘要: |
| 目的：仿制一种适合康复机制研究的局灶性颅脑外伤(TBI)动物模型，为国内广泛开展颅脑损伤康复机制研究提供基础。方法：成年雄性SD大鼠16只，随机分为颅脑外伤组和假手术组各8只。颅脑外伤组制作成控制性皮层损伤（CCI）模型，术后采用神经功能缺失评分、foot-fault test和cylinder test评估动物感觉和运动能力；外伤后第21～25天行Morris水迷宫检查，评估大鼠认知功能的变化；在外伤后28d通过焦油紫（CV）染色检测脑组织病理改变和组织缺失情况。结果：颅脑外伤组大鼠较假手术组神经功能评分有明显降低（P<0.05），运动协调能力和对称使用前肢的能力明显下降（P<0.05），空间学习和记忆能力明显损坏（P<0.05）；脑组织明显缺损（P<0.05），缺损组织周边出现神经元变性、坏死，神经元丢失伴胶质细胞增生。结论：控制性皮层损伤的TBI模型大鼠表现明显的感觉、运动、认知功能障碍和相应的病理改变，可以作为一项良好的TBI模型用于康复训练研究。 |
| 英文摘要: |
| Objective:To establish an animal experimental focal traumatic brain injury (TBI) model for investigating the underlying mechanisms of rehabilitation.Methods:After adult SD rats had received a severe controlled cortical impact (CCI) injury,they were randomly assigned to TBI group (n=8) or sham operation group (n=8).After TBI,the neurological deficits scoring,foot-fault test and cylinder test were performed.On 21-25 days post-injury,rats were tested for spatial learning and memory in a Morris Water Maze.On 28 days post-injury,animals were sacrificed and evaluated for quantitative cortical lesion volume and pathological changes.Results:The rats in TBI group demonstrated declined neurological deficits scores,coordination and asymmetry compared to sham operation group (P<0.05).The rats in TBI group showed obvious deficits in spatial learning and memory (P<0.05).We also observed significant change in lesion volume,neuron loss and overexpression of microglia around the lesion in TBI group (P<0.05).Conclusion:Rats undergoing a severe CCI injury showed significant sensorimotor,cognitive performance impairment and pathological changes.It is suggest that the model should be useful for assessing the effects of rehabilitation in TBI. |
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