| 蔡璇,许菁,徐姣,潘邓记.电针百会穴对小鼠大脑中动脉栓塞的保护作用研究[J].中国康复,2015,30(2):88-89 |
| 电针百会穴对小鼠大脑中动脉栓塞的保护作用研究 |
| Protective effects of electroacupuncture on Baihui on permanent middle cerebral artery occlusion |
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| DOI： |
| 中文关键词: 电针 小鼠 脑梗死 BK-channel |
| 英文关键词: electroacupuncture mice cerebral infarction BK-channel |
| 基金项目:国家自然基金（81371222） |
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| 中文摘要: |
| 目的：研究电针百会穴是否对小鼠局灶性脑梗死具有保护作用。方法: 将雄性昆明小鼠48只随机分为电针组和针刺组各24只，均通过颈内动脉线栓法建立小鼠大脑中动脉阻塞模型，电针组给予电针百会穴治疗，针刺组给予针刺百会穴治疗。治疗4周后，评估2组小鼠神经功能评分，测量梗塞体积，免疫组化检测超大B细胞淋巴瘤因子（Bcl-xl）的表达，对梗塞周边区域进行膜片钳检查。结果:电针组小鼠的梗死体积及神经功能评分均明显低于针刺组小鼠（P＜0.01）,Bcl-xl的表达较针刺组小鼠明显增加（P＜0.01）；脑片膜片钳结果显示，电针组小鼠BK-channel的功能明显好于针刺组（P＜0.01）。结论：电针百会穴能够降低小鼠脑梗死的体积并改善小鼠的神经功能，这种保护作用可能与电针刺激Bcl-xl的表达增加有关，也与神经元BK-channel离子通道的功能改善有关。 |
| 英文摘要: |
| Objective: To study the protective effect of electroacupuncture (EA) on Baihui on mice model of permanent occlusion of middle cerebral artery (MCAO). Methods: The MCAO model of mice were established and subjected to cerebral infarction by intraluminal occlusion of the middle cerebral artery. After intervention for 4 weeks, the lesion volume, the neurological impairment scores and the expression of Bcl-xl were observed and compared between the EA group and acupuncture group (psydoacupuncture). Patch clamp method was used in the the surrounding area of the infarction, and the change of BK-channel was observed. Result: The lesion volume and the neurological impairment scores in EA group were significantly reduced as compare with those in acupuncture group (P<0.01). The expression of Bcl-xl was higher in EA group than in acupuncture group (P<0.01). Patch clamp results showed that the BK-channel function of the neurons in EA group was significantly better than in acupuncture group in the surrounding area of the infarction (P<0.01). Conclusion: EA on Baihui can reduce the lesion volume and improve neurological function probably by up-regulating the expression of Bcl-xl and also by improving the function of neurons BK-channel. |
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