| 龚文辉,张素梅,储珏.有氧运动对饮食诱导的肥胖鼠下丘脑和褐色脂肪BMP7表达的影响[J].中国康复,2016,31(2):114-117 |
| 有氧运动对饮食诱导的肥胖鼠下丘脑和褐色脂肪BMP7表达的影响 |
| Effects of aerobic exercise on BMP7 protein expression in hypothalamus and brown adipose tissue in diet-induced obese rats |
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| DOI： |
| 中文关键词: 有氧运动 骨形成蛋白7 褐色脂肪 产热作用 |
| 英文关键词: aerobic exercise bone morphogenetic proteins 7 brown adipose tissue heat production |
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| 中文摘要: |
| 目的：研究8周有氧运动对饮食诱导的肥胖鼠下丘脑和棕色脂肪骨形成蛋白7（BMP7）蛋白表达、下丘脑产热功能调节酶腺苷酸(AMP)依赖的腺苷酸活化蛋白激酶(AMPK)表达，线粒体产热标志物环氧化酶2(COX2)表达的影响，以及机体产热功能的变化情况，探索运动影响棕色脂肪产热功能的机制。方法：SD雄性大鼠采用高脂肪饮食6周制备饮食诱导的肥胖(DIO)模型，分为DIO有氧运动组（EO组）、DIO安静对照组（CO组）和正常安静对照组（C组），3组各10只。DIO有氧运动组予8周(实验第14周)有氧跑台运动干预。免疫印记法检测下丘脑和棕色脂肪BMP 7、COX 2蛋白表达；封闭式流体压力呼吸计检测结算静止代谢率和非颤抖性产热。常规检测体质量、脂体比。结果：实验第6周，EO组及CO组大鼠体质量、脂体比及AMPK蛋白表达均显著高于C组（P<0.01）, RMR、NST、褐色脂肪BMP 7、COX 2蛋白表达及下丘脑BMP7蛋白表达均明显低于C组（P<0.01）；实验第14周，EO组大鼠体质量、脂体比及AMPK蛋白表达明显低于CO组（P<0.01），RMR、NST、大鼠褐色脂肪BMP 7、COX 2蛋白表达、下丘脑BMP7蛋白表达明显高于CO组（P<0.01），AMPK蛋白明显低于CO组（P<0.01）。结论：8周有氧运动能够通过增加肥胖鼠下丘脑、棕色脂肪BMP7表达，促进棕色脂肪产热，影响全身能量代谢，减轻体重。 |
| 英文摘要: |
| Objective:To explore the heat production mechanism of diet-induced obese (DIO) rats after 8-week aerobic exercise by observing the expression of BMP7, AMPK and COX2 in the hypothalamus and brown adipose tissue. Methods:The model of DIO SD male rats was established. DIO exercise group exerted 8-week aerobic exercise. BMP7 and COX2 protein expression was detected by Western blotting. Adipose/body mass, body mass, RMR, and NST were detected too. Results: BMP7 protein expression in the hypothalamus and brown adipose tissue was significantly increased in DIO exercise group as compared with obesity quiet group. As well as, COX2 protein expression was increased in DIO exercise group. Adipose/body mass, body mass, RMR, and NST in DIO exercise group were significantly decreased as compared with obesity quiet group. Conclusions: Eight-week aerobic exercise could facilitate brown adipose tissue heat production by increasing BMP7 protein expression in the hypothalamus and brown adipose tissue of DIO rats and adjusting energy metabolism function. |
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