文章摘要
张靖慧,方莹莹,李娜.rTMS对脑梗死大鼠记忆功能及海马IL-1的影响研究[J].中国康复,2016,31(3):165-167
rTMS对脑梗死大鼠记忆功能及海马IL-1的影响研究
Effects of rTMS on recovery of memory function and interleukin-1 in hippocampus after focal cerebral ischemia
  
DOI:
中文关键词: 经颅磁刺激  脑缺血  空间记忆  IL-1β
英文关键词: transcranial magnetic stimulation  cerebral ischemia  spatial memory  interleukin-1
基金项目:广东省自然科学基金博士启动项目(S2013040013489)
作者单位
张靖慧 中山大学附属第三医院康复医学科广州 510630 
方莹莹 南方医科大学基础医学院神经生物教研室广州 510630 
李娜 中山大学附属第三医院康复医学科广州 510630 
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中文摘要:
  目的:研究重复经颅磁刺激(rTMS)对脑梗死大鼠空间记忆功能的影响,并探讨其可能机制。方法:45只大鼠随机分为假手术组、对照组和rTMS组各15只,采用大脑中动脉栓塞再灌注(tMCAO)法制作脑梗死大鼠模型。采用Morris水迷宫评定学习记忆功能变化,通过实时定量聚合酶链式反应(qRT-PCR)观察患侧海马白细胞白介素1β(IL-1β)、诱导型一氧化氮合酶(iNOS)、肿瘤坏死因子(TNF-α)以及梗死灶区(IL-1β)mRNA表达量。 结果:与假手术组相比,对照组及rTMS组大鼠逃避潜伏期显著延长(P<0.01),而rTMS组较对照组的逃避潜伏期则明显缩短(P<0.05)。对照组、rTMS组IL-1β mRNA在患侧海马及皮层的表达量均较假手术组增高(P<0.01),rTMS组患侧海马IL-1β mRNA表达较对照组显著增高(P<0.01),患侧皮层IL-1β mRNA表达较对照组无明显变化。对照组、rTMS组iNOS、TNF-α mRNA表达量均较假手术组增高(P<0.01),rTMS组与对照组无明显差异。结论:rTMS治疗可促进脑梗死后空间学习记忆功能恢复,并可能通过增加患侧海马区IL-1βmRNA表达来实现。
英文摘要:
  Objective: To investigate the effects of rTMS in high frequency on the recovery of spatial memory function and the expression of interleukin-1beta (IL-1β) mRNA in ipsilesional hippocampus of rats subjected to focal cerebral ischemia. Methods: A rat model of acute focal cerebral ischemia was established using transient middle cerebral artery occlusion technique. Forty-five rats were randomly assigned to sham operative group, ischemic control group and rTMS group. Spatial memory function was evaluated by Morris water maze. The expression of pro-inflammatory cytokines (IL-1β, iNOS, TNF-α) in ipsilesional hippocampus and ischemic core was detected by real-time quantitative polymerase chain reaction. Results: The escape latency in ischemic control group and rTMS group was delayed more than that in sham operative group (P<0.01). The escape latency in rTMS group was significantly shortened as compared with that in model control group (P<0.05). The expression of IL-1β, iNOS and TNFα mRNA was increased in ischemic group and rTMS group (P<0.01) as compared with sham operative group (P<0.01). In the hippocampus, the expression of IL-1β mRNA in rTMS group was significantly increased as compared with that in the ischemic control group (P<0.01). In the ipsilateral cortex, there was no significant difference in the expression of IL-1β mRNA between rTMS group and ischemic control group. Conclusion: rTMS can promote the recovery of spatial memory function, which was possibly achieved by increasing the expression of IL-1β mRNA in the ipsilateral hippocampus.
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