| 王娟,郝赤子,廖维靖,张璇,万芪.“形神共养”对MCAO大鼠学习记忆能力及BDNF表达的影响[J].中国康复,2017,32(2):91-94 |
| “形神共养”对MCAO大鼠学习记忆能力及BDNF表达的影响 |
| Effect of "preservation from both physique and spirit" on learning and memory ability and expression of BDNF after focal ischemia in rats |
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| DOI： |
| 中文关键词: 丰富生存环境 形神共养 脑缺血再灌注 脑源性神经营养因子 |
| 英文关键词: enriched environment preservation from both physique and spirit cerebral ischemia reperfusion brain-derived neurotrophic factor |
| 基金项目:国家自然科学基金项目（81173315） |
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| 中文摘要: |
| 目的：探讨“形神共养”的丰富生存环境对大脑中动脉阻塞(MCAO)大鼠学习记忆能力以及抗大鼠脑源性神经营养因子(BDNF)表达的影响。方法：将50只SD雄性大鼠随机分为缺血“形养”组（IE1）、缺血“神养”组（IE2）、缺血“形神共养”组（IE3）、缺血标准环境组（IS）、假手术标准环境组（SS），每组10只。缺血组进行右侧大脑中动脉阻断及再灌注手术。术后进行为期4周的“形神共养”干预，干预后进行水迷宫实验，实验结束后用免疫组织化学染色观察脑梗死周边皮质及海马齿状回区BDNF的表达。结果：在水迷宫空间探索实验中，大鼠的逃避潜伏期逐渐缩短，训练第5天时，IE1、IE3组逃避潜伏期均比IS组短（均P＜0.05），且IE1、IE3组与SS组之间无显著性差异；IE1、IE2、IE3组大鼠对原平台的记忆均比IS好。IE1、IE3组梗死灶周围皮质BDNF蛋白的表达比IS组高；在缺血侧海马DG部位，IE1、IE2、IE3组BDNF蛋白的表达均比IS、SS组高（均P＜0.05）。结论：“形神共养”的丰富生存环境有利于促进局灶性脑缺血再灌注大鼠学习记忆能力的恢复；有利于促进局灶性脑缺血再灌注大鼠梗死周边皮质和海马齿状回区BDNF的表达，从而促进脑缺血大鼠功能的恢复。 |
| 英文摘要: |
| Objective: To investigate the impact of "preservation from both physique and spirit" on cognitive ability and brain-derived neurotrophic factor (BDNF) after focal ischemia in rats. Methods: Fifty Sprague-Dawley rats were divided into five groups: ischemic rats housed in the enriched environment of "physique" (IE1), ischemic rats housed in the enriched environment of "spirit" (IE2), ischemic rats housed in the enriched environment of "preservation from both physique and spirit" (IE3), ischemic rats housed in standard cages (IS), and sham-ischemic rats in standard cages (SS) (n=10 in each group). Rats in the ischemia/reperfusion groups had their middle cerebral artery sutured for two h before reperfusion, and rats were placed in a "preservation from both physique and spirit" enriched-environment for 4 weeks. The water Maze test was used to assess spatial learning and memory after environmental intervention. Then the rats were sacrificed. BDNF immunoreactivity was quantified in peri-infarct area and hippocampus dentate gyrus. Results: The escape latency was significantly shorter in groups IE1 and IE3 than in group IS on the 5th days (P<0.05), but there was no significant difference among IE1, IE3 and SS groups. The time spent in the target quadrant was significantly longer in IE1, IE2 and IE3 groups than in IS group. The BDNF protein levels in the peri-lesional cortex in groups IE1 and IE3 were higher than in IS group, and those in hippocampus dentate gyrus in IE1, IE2 and IE3 groups were higher than in IS and SS groups (P<0.05). Conclusions: We conclude that "preservation from both physique and spirit" enriched-environment housing has positive effects on learning and memory ability, and increases BDNF levels in rats after focal ischemia. |
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