| 赵晨光,许刚,琚芬,孙玮,袁华,牟翔.LINGO-1在脊髓神经干细胞向少突胶质细胞分化中的作用研究[J].中国康复,2017,32(3):185-188 |
| LINGO-1在脊髓神经干细胞向少突胶质细胞分化中的作用研究 |
| Effects of LINGO-1 on oligodendrocyte differentiation of spinal cord-derived neural stem cells |
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| DOI： |
| 中文关键词: LINGO-1 脊髓神经干细胞 少突胶质细胞 分化 |
| 英文关键词: LINGO-1 spinal cord-derived neural stem cells oligodendrocyte differentiation |
| 基金项目:国家自然科学基金（81100932）；中国博士后科研基金（20100481518）；科技部国际合作重点专项基金（2013DFA32610） |
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| 中文摘要: |
| 目的：观察LRR结构和免疫球蛋白结构域Nogo受体作用蛋白（LINGO-1）在脊髓神经干细胞(SpNSCs)向少突胶质细胞分化过程中的表达特征及其对少突胶质细胞分化成熟的作用。方法：体外培养大鼠脊髓神经干细胞并诱导分化，于分化第3天及第5天进行LINGO-1与A2B5及O4免疫荧光双染色，观察LINGO-1的表达特征。体外培养的大鼠脊髓来源神经干细胞分为对照组及干扰组，干扰组通过LINGO-1 shRNA慢病毒感染SpNSCs下调LINGO-1表达，对照组表达Scramble-shRNA的慢病毒，通过LINGO-1 shRNA慢病毒感染SpNSCs下调LINGO-1表达，于分化第7天免疫荧光染色检测少突胶质细胞分化情况及髓鞘碱性蛋白（MBP）表达情况。结果：LINGO-1与A2B5及O4均共表达于分化细胞中。下调LINGO-1表达后，成熟少突胶质细胞达到对照组细胞的3.28倍，MBP表达量为对照组的5.31倍，且均具有统计学意义（均P<0.05）。结论：LINGO-1在少突胶质细胞分化过程中持续表达，LINGO-1负性调控少突胶质细胞分化及成熟。 |
| 英文摘要: |
| Objective: To investigate the expression of LINGO-1 in the oligodendrocyte differentiation of spinal cord-derived neural stem cells (SpNSCs). Methods: SpNSCs were isolated from rat spinal cord and cultured in vitro. The expression of LINGO-1 was detected by double immunofluorescence staining of LINGO-1 and A2B5, O4 at 3th and 5th day of differentiation. SpNSCs isolated from rat spinal cord were cultured in vitro and divided into RNAi group and control group. The RNAi group was transfected with LINGO-1 shRNA lentiviral vector to down-regulate the expression of LINGO-1, and control group was transfected with lentiviral vector. The oligodendrocyte was examined by immunofluorescence staining and MBP expression was detected using Western blotting at 7th day of differentiation. Results: LINGO-1 was co-expressed with A2B5 and O4. After down-regulation of LINGO-1 expression, the number of mature oligodendrocytes was 3.28 fold over the control group, and the expression of MBP was 5.31 fold over the control group (P<0.05 for all). Conclusion: LINGO-1 was constantly expressed and had a negative effect on the differentiation of oligodendrocytes. |
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