| 缪吉,雷涛,杨光显,金丽.三七皂苷对运动性贫血大鼠海马BDNF表达的影响[J].中国康复,2017,32(6):484-487 |
| 三七皂苷对运动性贫血大鼠海马BDNF表达的影响 |
| Effects of PNS on the Hippocampal BDNF expression in sports anemia rats |
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| DOI： |
| 中文关键词: 运动性贫血 三七皂苷 海马 BDNF |
| 英文关键词: sports anemia PNS Hippocampal BDNF |
| 基金项目:霍英东教育基金会青年教师基金基础性研究项目（121103） |
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| 中文摘要: |
| 目的：探讨运动性贫血对大鼠海马BDNF表达的影响以及三七皂苷在运动性贫血发生发展过程中对大鼠神经系统的作用。方法：将24只健康雄性4周龄SD大鼠随机分为对照组、运动组和灌药组各8只。对运动组和灌药组大鼠进行为期10周的递增负荷跑台运动，并从第5周开始对灌药组大鼠进行三七皂苷药物干预。应用Real-time PCR检测海马组织BDNF mRNA的基因表达情况；应用ELISA测定海马组织BDNF的蛋白含量。结果：10周训练后，运动组大鼠海马BDNF mRNA含量及BDNF蛋白含量测定均较对照组及灌药组低（P<0.05），灌药组与对照组比较差异无统计学意义。结论：运动性贫血导致大鼠脑部发生缺血缺氧性损伤，三七皂苷能促进大鼠海马BDNF的表达，对运动性贫血造成的脑缺血缺氧性损伤发挥保护作用。 |
| 英文摘要: |
| Objective: To investigate the effects of sports anemia on the expression of BDNF in rat hippocampi and the roles of PNS in rat nervous system in the sports anemia development. Methods: Twenty-four healthy male 4-week-old SD rats were randomly divided into control group, sports group and PNS intervention group. The rats in both sports group and PNS intervention group were subjected to the increasing load treadmill exercise for 10 weeks, and those in the PNS intervention group were given drug intervention from the fifth week additionally. Hippocampal BDNF mRNA gene expression was detected using real time PCR, and BDNF protein content was determined by ELISA. Results: After ten weeks increasing load treadmill training, the hippocampal BDNF mRNA and protein expression in sports group was significantly lower than in control group and PNS intervention group (P<0.05). Both BDNF mRNA and protein expression showed no significant difference between PNS intervention group and control group. Conclusion: These results suggest that sports anemia may induce brain hypoxic-ischemic injury in rats. The PNS can promote the expression of BDNF in rat hippocampi, which may play a protective role in sports anemia-induced brain hypoxic-ischemic injury. |
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