| 龙兴蓝,许涛,邹丽丽,蒋婷,彭涛,孙衢骎.Wnt/β-catenin通路在脉冲强磁场促神经干细胞增殖中的作用初探[J].中国康复,2018,33(4):267-271 |
| Wnt/β-catenin通路在脉冲强磁场促神经干细胞增殖中的作用初探 |
| Role of Wnt/β-catenin pathway in proliferation of neural stem cells induced by high-intensity pulsed electromagnetic fields |
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| DOI： |
| 中文关键词: 神经干细胞 脉冲强磁场 增殖 RNAi wnt/β-catenin通路 |
| 英文关键词: Neural stem cells High-intensity pulsed electromagnetic fields Proliferation RNAi Wnt/β-catenin pathway |
| 基金项目:国家自然科学基金（面上项目）（81271732） |
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| 摘要点击次数: 8290 |
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| 中文摘要: |
| 目的：摸索抑制神经干细胞（NSCs）中β-catenin表达的合适条件，为获得抑制β-catenin表达的NSCs做准备。利用抑制β-catenin表达的NSCs，探索wnt/β-catenin通路在脉冲强磁场促NSCs增殖中的作用。方法：取新生的SD大鼠双侧侧脑室下的NSCs，然后用无血清培养基培养14d。利用RNAi原理构建β-catenin shRNA慢病毒载体及阴性慢病毒载体，并转染NSCs，确定合适的感染复数(MOI)值。将NSCs分为阴性组（阴性病毒转染组）和实验组（β-catenin shRNA病毒转染组），并用4T、0.1Hz、8次的脉冲强磁场干预。在用脉冲强磁场干预后的1d、7d，采用CCK8法检测阴性组和实验组的NSCs增殖情况。结果：β-catenin shRNA序列在MOI=10时，对NSCs中β-catenin的抑制效果最好。阴性病毒组在脉冲强磁场干预后1d、7d 时NSCs增殖最明显（P＜0.05），而实验组在干预前后NSCs增殖水平无明显变化。结论：wnt/β-catenin通路在脉冲强磁场促NSCs增殖中起作用。 |
| 英文摘要: |
| Objective: To investigate the optimal conditions of inhibiting the β-catenin expression in neural stem cells (NSCs) for harvesting NSCs low-expressing β-catenin, and exploring the roles of Wnt/β-catenin pathway in proliferation of NSCs induced by the high-intensity pulsed electromagnetic fields. Methods: (1) NSCs were derived from subventricular zone (SVZ) of newborn SD rats, and then cultured for 14 days using serum-free medium. (2) The RNAi method was used to inhibit β-catenin expression in NSCs, and the optimal MOI for transfection to inhibit β-catenin levels in NSCs was measured. (3) The NSCs were divided into the negative control group and experimental group, and then they were exposed in the high-intensity pulsed electromagnetic fields with stimulus condition of 4T, 0.1Hz, 8 times. (4) At 1st and 7th day after the intervention with the high-intensity pulsed electromagnetic fields, the proliferation of NSCs was tested by the CCK8 assay. Results: (1) When MOI=10, the best inhibitory effects on β-catenin could be obtained in NSCs. (2) At 1st and 7th day after NSCs were exposed to the high-intensity pulsed electromagnetic fields, the proliferation of NSCs in the negative control group was significantly higher than in the experimental group (P<0.05). Conclusion: The Wnt/β-catenin pathway may play a role in proliferation of NSCs induced by the high-intensity pulsed electromagnetic fields. |
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