| 谢冰,肖乐,刘超,李元,孔瑛.游泳运动对大鼠膝关节骨关节炎内质网应激PERK表达的影响[J].中国康复,2018,33(5):355-359 |
| 游泳运动对大鼠膝关节骨关节炎内质网应激PERK表达的影响 |
| Effect of swimming exercise on expression of protein kinase R-like endoplasmic reticulum kinase (PERK) as a signaling pathway of endoplasmic reticulum stress in rats with knee osteoarthritis |
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| DOI： |
| 中文关键词: 游泳运动 骨关节炎 PERK 内质网应激 |
| 英文关键词: Swimming Osteoarthritis protein kinase R-like endoplasmic reticulum kinase Endoplasmic reticulum stress |
| 基金项目:国家自然科学基金项目（81201503） |
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| 中文摘要: |
| 目的：通过研究游泳运动对内质网应激PERK表达的影响，探讨游泳运动治疗膝关节骨关节炎可能的作用机制。方法：将雄性健康SD大鼠24只随机分为空白对照组（A组）、假手术组（B组），模型对照组（C组）和游泳运动组（D组），每组6只。A组笼内自由活动8周，B组假手术后笼内自由活动8周，C组造模后笼内自由活动8周，D组造模后笼内自由活动4周后进行游泳训练4周。治疗结束后处死大鼠，观察关节内情况，并进行大体评分，行HE染色及甲苯胺蓝染色，进行改良Mankin评分，免疫组化法检测PERK的表达。结果：大体观察及评分、HE染色、甲苯胺蓝染色显示C、D组关节软骨损伤程度较A组严重，C、D组与A组评分比较明显增高（P<0.05）；B组与A组比较差异无统计学意义；D组与C组比较评分降低（P<0.05）。PERK的表达C、D组较A、B组增高（P<0.05）；D组较C组PERK阳性细胞数降低（P<0.05）；B组与A组比较差异无统计学意义。结论：游泳运动可对膝关节骨关节炎起治疗作用，其作用机制之一可能是通过减少PERK的表达，降低内质网应激，减少软骨细胞凋亡实现的。 |
| 英文摘要: |
| Objective: To study the possible mechanism of swimming exercise for knee osteoarthritis by investigating the expression of swimming exercise on the expression of protein kinase R-like endoplasmic reticulum kinase (PERK) as a signaling pathway of endoplasmic reticulum stress. Methods: Sprague Dawley rats were randomly divided into a normal group (group A), sham operation group (group B), model control group (group C), and swimming exercise group (group D). Rats in group A were allowed for free activities in their cage for 8 weeks, and rats in groups B and C were allowed for free activities in their cage for 8 weeks after the sham operation and experimental operation, respectively. Rats in group D were allowed for free activities for 4 weeks after the operation and then underwent swimming exercises for 4 weeks. The cartilage changes, hematoxylin-eosin (HE) staining, toluidine blue staining, immunohistochemical staining, Mankin’s grade, gross scores, and the PERK contents in chondrocytes were compared among the groups. Results: Cartilage degradation was more severe in groups C and D than in group A as shown by gross observation, gross scores, HE staining, and toluidine blue staining (P<0.05). No significant differences were noted between groups A and B. The changes in group D were significantly less severe than those in group C (P<0.05). Immunohistochemical staining showed that the expression of PERK in articular cartilage was higher in groups C and D than in groups A and B (P<0.05), and lower in group D than in group C (P<0.05). Conclusion: Swimming exercise can promote the repair of articular cartilage possibly by reducing the expression of PERK, endoplasmic reticulum stress, and apoptosis rate of chondrocytes. |
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