文章摘要
谢冰,肖乐,刘超,李元,孔瑛.游泳运动对大鼠膝关节骨关节炎内质网应激PERK表达的影响[J].中国康复,2018,33(5):355-359
游泳运动对大鼠膝关节骨关节炎内质网应激PERK表达的影响
Effect of swimming exercise on expression of protein kinase R-like endoplasmic reticulum kinase (PERK) as a signaling pathway of endoplasmic reticulum stress in rats with knee osteoarthritis
  
DOI:
中文关键词: 游泳运动  骨关节炎  PERK  内质网应激
英文关键词: Swimming  Osteoarthritis  protein kinase R-like endoplasmic reticulum kinase  Endoplasmic reticulum stress
基金项目:国家自然科学基金项目(81201503)
作者单位
谢冰 长沙市第一医院康复医学科长沙 410001 
肖乐 长沙市第一医院康复医学科长沙 410001 
刘超 长沙市第一医院康复医学科长沙 410001 
李元 长沙市第一医院康复医学科长沙 410001 
孔瑛 中南大学湘雅二医院长沙 410001 
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中文摘要:
      目的:通过研究游泳运动对内质网应激PERK表达的影响,探讨游泳运动治疗膝关节骨关节炎可能的作用机制。方法:将雄性健康SD大鼠24只随机分为空白对照组(A组)、假手术组(B组),模型对照组(C组)和游泳运动组(D组),每组6只。A组笼内自由活动8周,B组假手术后笼内自由活动8周,C组造模后笼内自由活动8周,D组造模后笼内自由活动4周后进行游泳训练4周。治疗结束后处死大鼠,观察关节内情况,并进行大体评分,行HE染色及甲苯胺蓝染色,进行改良Mankin评分,免疫组化法检测PERK的表达。结果:大体观察及评分、HE染色、甲苯胺蓝染色显示C、D组关节软骨损伤程度较A组严重,C、D组与A组评分比较明显增高(P<0.05);B组与A组比较差异无统计学意义;D组与C组比较评分降低(P<0.05)。PERK的表达C、D组较A、B组增高(P<0.05);D组较C组PERK阳性细胞数降低(P<0.05);B组与A组比较差异无统计学意义。结论:游泳运动可对膝关节骨关节炎起治疗作用,其作用机制之一可能是通过减少PERK的表达,降低内质网应激,减少软骨细胞凋亡实现的。
英文摘要:
      Objective: To study the possible mechanism of swimming exercise for knee osteoarthritis by investigating the expression of swimming exercise on the expression of protein kinase R-like endoplasmic reticulum kinase (PERK) as a signaling pathway of endoplasmic reticulum stress. Methods: Sprague Dawley rats were randomly divided into a normal group (group A), sham operation group (group B), model control group (group C), and swimming exercise group (group D). Rats in group A were allowed for free activities in their cage for 8 weeks, and rats in groups B and C were allowed for free activities in their cage for 8 weeks after the sham operation and experimental operation, respectively. Rats in group D were allowed for free activities for 4 weeks after the operation and then underwent swimming exercises for 4 weeks. The cartilage changes, hematoxylin-eosin (HE) staining, toluidine blue staining, immunohistochemical staining, Mankin’s grade, gross scores, and the PERK contents in chondrocytes were compared among the groups. Results: Cartilage degradation was more severe in groups C and D than in group A as shown by gross observation, gross scores, HE staining, and toluidine blue staining (P<0.05). No significant differences were noted between groups A and B. The changes in group D were significantly less severe than those in group C (P<0.05). Immunohistochemical staining showed that the expression of PERK in articular cartilage was higher in groups C and D than in groups A and B (P<0.05), and lower in group D than in group C (P<0.05). Conclusion: Swimming exercise can promote the repair of articular cartilage possibly by reducing the expression of PERK, endoplasmic reticulum stress, and apoptosis rate of chondrocytes.
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