文章摘要
于秋红,李冬果,张琪,任梓齐,嵇江淮,刘亚玲,王丛,薛连璧.高压氧对人胶质瘤172细胞生存的影响[J].中国康复,2020,35(6):283-286
高压氧对人胶质瘤172细胞生存的影响
Effect of hyperbaric oxygen on survival of human glioma 172 cells
  
DOI:
中文关键词: 高压氧  胶质瘤  172细胞  凋亡  超微结构
英文关键词: hyperbaric oxygen  glioma  172 cells  apoptosis  ultrastructure
基金项目:首都医科大学基础-临床科研合作基金(天坛专项)17JL(TTZX)10
作者单位
于秋红 首都医科大学附属北京天坛医院高压氧科北京 100070 
李冬果 首都医科大学生物医学工程学院北京 100069 
张琪 北京市神经外科研究所首都医科大学附属北京天坛医院电镜室北京 100070 
任梓齐 首都医科大学附属北京天坛医院高压氧科北京 100070 
嵇江淮 首都医科大学生物医学工程学院北京 100069 
刘亚玲 首都医科大学附属北京天坛医院高压氧科北京 100070 
王丛 首都医科大学附属北京天坛医院高压氧科北京 100070 
薛连璧 首都医科大学附属北京天坛医院高压氧科北京 100070 
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中文摘要:
  目的:观察高压氧(HBO)对人胶质瘤172细胞生存的影响。方法:常规培养的人胶质瘤172细胞随机分为对照组和HBO组,对照组每日实验舱中常压0.1MPa空气放置90min,其余时间正常培养,HBO组每日HBO暴露1次,将细胞置实验舱中,氧气加压,15min升压至0.2MPa,稳压1h,减压15min,其余时间培养同对照组,HBO暴露10次。第11d取材,观察2组细胞生长情况,流式细胞仪检测细胞凋亡比例、细胞生长周期,电镜观察细胞超微结构。结果:HBO组细胞计数明显少于对照组(P=0.01),HBO组早期和晚期凋亡细胞比例均明显高于对照组(均P<0.01) 。2组G1期和S期细胞比例比较差异均无统计学意义,HBO组G2期细胞比例较对照组明显减少(P<0.01)。电镜观察到HBO组肿瘤细胞较对照组突起短小、减少。结论:HBO减慢了人胶质瘤172细胞生长,促进细胞凋亡,使更多的细胞阻滞在S期,使与肿瘤的转移、复发有关的突起变短、减少,显示了HBO对肿瘤细胞的抑制作用。
英文摘要:
  Objective: To observe the effect of hyperbaric oxygen (HBO) on survival of human glioma 172 cells. Methods: Cultured human glioma 172 cells were randomly divided into control group and HBO group. The cells in control group were placed in chamber of 0.1 MPa for 90 min per day, and then cultured in 37℃ 5% CO2 incubator. The cells in HBO group were exposed to HBO with pressure increasing from 0.1 MPa to 0.2 MPa within 15 min, and maintaining at 0.2 MPa for 1 h, then decreasing to 0.1 MPa within 15 min per day, and then cultured in 37℃ 5% CO2 incubator. The cells were exposed to HBO 10 times. On the 11th day, cells of both groups were collected. Cell growth was observed under light microscopy, and apoptosis and cell growth cycle were detected by flow cytometry. The ultrastructure of cells was observed by electron microscopy. Results: The cell count of the HBO group was significantly less than that of the control group (P=0.01), and the proportion of early and late apoptotic cells in the HBO group was significantly higher than that in the control group (both P<0.01). There was no statistically significant difference in the proportion of G1 and S phase cells between the two groups. The proportion of G2 phase cells in the HBO group was significantly reduced as compared with the control group ( P<0.01). The protrusions of 172 cells in HBO group were shorter and smaller than those of control group. Conclusion: HBO can delay the growth of human glioma 172 cells, and promote apoptosis of 172 cells, with more cells blocking in the S phase, together with the protrusions shorten and reduced which related to tumor metastasis and recurrence, implying HBO has an inhibitory effect on human glioma 172 cells.
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