| 林璐,周甜甜,郝赤子,廖维靖.丰富环境对血管性痴呆大鼠神经炎症和反应性星形胶质细胞的影响[J].中国康复,2021,36(11):643-648 |
| 丰富环境对血管性痴呆大鼠神经炎症和反应性星形胶质细胞的影响 |
| Effects of enriched environment on neuroinflammation and reactive astrocytes in vascular dementia rats |
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| DOI： |
| 中文关键词: 丰富环境 血管性痴呆 神经炎症 反应性星形胶质细胞 |
| 英文关键词: enriched environment vascular dementia neuroinflammation reactive astrocytes |
| 基金项目:湖北省卫生计生委中医药科研项目（SZY2019003） |
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| 中文摘要: |
| 目的：探究丰富环境（EE）对血管性痴呆（VaD）大鼠海马区神经炎症和反应性星形胶质细胞的影响。方法：将36只成年雄性SD大鼠随机分为假手术组、模型组和模型+EE组各12只。用永久性双侧颈总动脉结扎术（2-VO）制备VaD模型。4周环境干预后，采用Morris水迷宫实验、原位末端转移酶标记（TUNEL）染色、实时定量聚合酶链反应（RT-qPCR）和免疫荧光双标法分别检测VaD大鼠的学习和记忆能力、海马神经元凋亡情况、炎症因子白细胞介素-1β（IL-1β）和反应性星形胶质细胞中神经胶质纤维酸性蛋白（GFAP）和脂质运载蛋白-2（LCN2）的表达变化。结果：模型+EE组在逃避潜伏期、目标象限所花时间和穿越平台次数上均表现得比模型组更好（P<0.05），但低于假手术组（P<0.05）。模型组大鼠神经元的TUNEL阳性细胞、IL-1β、GFAP和LCN2明显多于其余2组（P<0.05），除GFAP以外，假手术组TUNEL阳性细胞、IL-1β和LCN2的表达均显著低于模型+EE组（P<0.05）。结论：EE能减轻海马神经炎症和反应性星形胶质细胞的激活，同时伴随着VaD大鼠的认知功能的改善。 |
| 英文摘要: |
| Objective: To investigate the effect of enriched environment (EE) on the hippocampus neuroinflammation and reactive astrocytes of vascular dementia (VaD) rats. Methods: Totally, 36 adult male SD rats were randomly divided into sham operation group, model group and model+EE group with 12 rats in each group. 2-vessel Occlusion (2-VO) surgery was used to make the rat model of VaD. After 4 weeks of environmental intervention, Morris water maze experiment, terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end labeling (TUNEL) staining, real-time quantitative polymerase chain reaction (RT-qPCR), and double immunofluorescence labeling were used to detect the changes of learning and memory ability, the hippocampus neuron apoptosis, interleukin-1β (IL-1β) and glial fibers in acidic protein (GFAP) and lipocalin-2 (LCN2) in reactive astrocytes of VaD rats. Results: The model+EE group performed better in escape latency, the time spent in target quadrant and the number of crossing platform than the model group (P<0.05), but still not as good as the sham group (P<0.05). The TUNEL positive cells, IL-1β, GFAP and LCN2 in the model group were significantly increased as compared with those in the rest two groups (P<0.05). The expression levels of TUNEL positive cells, IL-1β and LCN2 in the sham group were significantly lower than those in the model+EE group (P<0.05), except for GFAP. Conclusion: EE can reduce hippocampus neuroinflammation and the activation of reactive astrocytes, accompanied by improved cognitive function in VaD rats. |
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