| 王风波,唐明薇,王科,王琼芬,王宝兰.早期高压氧联合康复治疗对脑出血自噬蛋白Beclin-1的影响[J].中国康复,2021,36(12):707-711 |
| 早期高压氧联合康复治疗对脑出血自噬蛋白Beclin-1的影响 |
| Effect of hyperbaric oxygen combined with early rehabilitation training on autophagy protein Beclin-1 in intracerebral hemorrhage |
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| DOI： |
| 中文关键词: 高压氧 早期康复 脑出血 Beclin-1 行为学 |
| 英文关键词: hyperbaric oxygen early rehabilitation cerebral hemorrhage Beclin-1 ethology |
| 基金项目:成都医学院四川应用心理学研究中心资助项目（CSXL-193005） |
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| 中文摘要: |
| 目的:探讨高压氧联合早期康复训练对脑出血大鼠海马区脑细胞自噬相关蛋白Beclin-1表达及行为学的影响作用。方法：55只成年雄性SD大鼠按随机数字表法分为空白组、非干预模型组、高压氧组、康复训练组及联合干预组，每组各10只，备用大鼠5只。非干预模型组、高压氧组、康复训练组、联合干预组大鼠采用胶原酶Ⅶ注射建立脑出血模型，高压氧组、康复训练组均于术后第3天分别开始行高压氧、康复训练，联合干预组包括以上干预。干预第7天及第14天，各组随机取5只大鼠行神经行为学Longa评分，评分完毕取海马区行免疫组化法Beclin-1检测。结果：干预7d及14d，高压氧组、康复训练组、联合干预组大鼠Longa评分较非干预模型组同时间点比较均明显下降（均P＜0.05），联合干预组Longa评分均更低于高压氧组和康复训练组（均P＜0.05）。高压氧组、康复训练组、联合干预组大鼠海马区Beclin-1阳性反应较非干预模型组同时间点比较均明显下降（均P＜0.05），联合干预组的下降幅度均大于高压氧组及康复训练组（均P＜0.05）。结论：高压氧及康复训练均可有效调节脑出血早期脑细胞自噬相关蛋白的表达，而高压氧联合早期康复的调节作用优于单纯高压氧和康复训练，脑出血后神经行为学的改善可能与脑的自噬相关蛋白调控相关。 |
| 英文摘要: |
| Objective: To explore the effect of hyperbaric oxygen combined with early rehabilitation training on the expression of autophagy related protein Beclin-1 in hippocampus of rats with intracerebral hemorrhage and their behaviors. Methods：A total of 55 adult male SD rats were randomly divided into blank group, non-intervention model group, hyperbaric oxygen group, rehabilitation training group and combined intervention group, 10 rats in each group, and 5 rats were used for standby. Rats in non-intervention model group, hyperbaric oxygen group, rehabilitation training group and combined intervention group were injected with collagenase Ⅶ to establish cerebral hemorrhage model. Hyperbaric oxygen group and rehabilitation training group were given hyperbaric oxygen and rehabilitation training respectively on the 3rd day after operation, and the combined intervention group was subjected to the above interventions. On the 7th and 14th days after the intervention, 5 rats from each group were randomly selected and scored by neurobehavioral Longa score. After scoring, Beclin-1 was detected in the hippocampus by immunohisto-chemical method. Results: Compared with the non-intervention model group, the Longa scores of rats in hyperbaric oxygen group, rehabilitation training group and combined intervention group were significantly decreased (all P<0.05), and that in the combined intervention group was lower than the hyperbaric oxygen group and rehabilitation training group (both P<0.05). As compared with the non-intervention model group, Beclin-1 positive reaction in hippocampus of rats in hyperbaric oxygen group, rehabilitation training group and combined intervention group was significantly decreased (all P<0.05). The decrease amplitude of combined intervention group was greater than that of hyperbaric oxygen group and rehabilitation training group (both P<0.05). Conclusion: Hyperbaric oxygen and early rehabilitation training can effectively regulate the expression of autophagy related proteins in brain cells in the early stage of cerebral hem-orrhage, and hyperbaric oxygen combined with rehabilitation training is superior to hyperbaric oxygen and rehabilitation training alone. The improvement of neurobehavior after intracerebral hemorrhage may be related to the regulation of autophagy-related proteins in the brain. |
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