| 柴源,王宏康,赵瑞婧,苗宇.间充质干细胞外泌体对MPTP诱导急性帕金森小鼠模型的治疗作用[J].中国康复,2022,37(9):515-518 |
| 间充质干细胞外泌体对MPTP诱导急性帕金森小鼠模型的治疗作用 |
| Therapeutic effect of mesenchymal stem cell derived-exosomes on MPTP-induced acute Parkinson’s model of mice |
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| DOI： |
| 中文关键词: 间充质干细胞 外泌体 帕金森疾病 |
| 英文关键词: mesenchymal stem cells exosomes Parkinson’s disease |
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| 中文摘要: |
| 目的：本文探讨间充质干细胞外泌体（MSCs-Exo）对小鼠帕金森疾病（PD）模型的治疗作用，为帕金森的治疗以及MSC-Exo的应用提供实验依据。方法：超速离心法提取MSC-Exo。将45只C57BL/6小鼠分为3组，分别为Sham组、PD组和PD+MSC-Exo组，每组15只，Sham组在造模时给予生理盐水，PD组腹腔注射1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP） 诱导小鼠急性PD模型，PD+MSC-Exo组在PD造模后尾静脉注射给予MSC-Exo。在第4、14和30天通过旋转实验评价小鼠的行为学变化，第30天通过尼氏染色观察神经元的损失情况，第4天通过免疫印迹检测酪氨酸羟化酶（TH）、NFE2相关因子2（Nrf2）、Kelch样环氧氯丙烷相关蛋白-1（keap1）、肿瘤坏死因子α（TNFα）、白细胞介素1β（IL-1β）等的表达，分析MSC-Exo对炎症的调控作用。结果：在4、14和30天时，PD+MSC-Exo组转圈数显著少于PD组（P<0.01）；在第4天时，与Sham组相比，PD组的尼氏阳性细胞数显著减少，而PD+MSC-Exo组相比PD组尼氏细胞显著增多；免疫印迹结果显示，相比Sham组，PD组的TH表达显著减少，Nrf2、keap1、TNFα、IL-1β表达显著增加（P<0.01），而PD+MSC-Exo组的TH相较于PD组明显增加，Nrf2、keap1、TNFα、IL-1β则显著降低（P<0.01）。结论：MSC-Exo能够保护神经元，减少PD模型中的神经元失去，减少炎症的发生，对PD模型具有良好的治疗作用。 |
| 英文摘要: |
| Objective:To explore the therapeutic effect of MSCs-derived exosomes (MSc-exo) on mouse Parkinson's disease（PD） model, so as to provide experimental basis for the treatment of PD and the application of MSC-exo. Methods: MSC-exo were extracted by ultracentrifugation, and identified by immunoblotting and transmission electron microscopy. C57BL/6 mice were divided into three groups: sham group, PD group and PD + MSC-exo group. C57BL/6 mice were injected intraperitoneally with MPTP to induce acute PD model. MSC-exo or PBS were injected into caudal vein. The behavioral changes of mice were analyzed by rotation experiment on the 4th, 14th and 30th days. Nissl staining and immunoblotting of Nrf2, HO1, TNFα and IL-1β were used to observe the effect of MSC-exo on PD model. Results: At 4th, 14th and 30th day, the number of turns in PD + MSc exo group was significantly less than that in PD group (P<0.0001). Compared with sham group, Nissl positive cells in PD group decreased significantly, while Nissl cells in PD + MSC-exo group increased significantly as compared with PD group. Western blot showed that as compared with sham group, TH expression in PD group decreased significantly, and Nrf2, keap1, TNFα and IL-1β increased significantly. The expression of TH increased significantly in PD + MSC-exo, but that of Nrf2, keap1, TNFα and IL-1β decreased significantly as compared with PD group. Conclusion: MSC-exo can protect neurons, decrease the loss of neurons in PD model and reduce the occurrence of inflammation. So, MSC-Exo have a satisfactory therapeutic effect on PD model. |
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