| 陈诺梅,陈海萍,成熟,李璇,孙锦文,张威.糖调节受损患者肠道菌群特征及高能量激光的干预作用探究[J].中国康复,2022,37(12):741-746 |
| 糖调节受损患者肠道菌群特征及高能量激光的干预作用探究 |
| Characteristics of intestinal microflora in patients with impaired glucose regulation and the intervention effect of high energy laser |
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| DOI： |
| 中文关键词: 糖调节受损 高能量激光 肠道菌群 |
| 英文关键词: Impaired glucose regulation High energy laser Intestinal flora |
| 基金项目:湖北省卫生健康委联合基金立项项目 (WJ2019H199) |
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| 中文摘要: |
| 目的：分析糖调节受损患者肠道菌群结构特征，并探讨高能量激光的干预作用。方法：将13例糖调节受损患者纳入观察组，并选取10名健康体检者作为对照组，观察组予以980nm半导体激光辐照腹部9个治疗位点，疗程1个月，提取观察组干预前后及对照组大便菌群基因组DNA进行高通量测序。结果：在门分类水平上，观察组干预后与干预前相比拟杆菌门相对丰度显著提高（P＜0.05）。在属分类水平上，与对照组相比，干预前观察组副杆菌属和粪杆菌属相对丰度显著降低(P＜0.05)；观察组干预后与干预前相比副杆菌属显著升高(P＜0.05)，链球菌属相对丰度显著下降(P＜0.05)。在物种多样性上，干预前观察组在多样性及均匀度上均显著小于对照组(P＜0.05)，观察组干预后与干预前相比在丰富度、多样性及均匀度上均得到显著提高(P＜0.05)。结论：糖调节异常患者肠道菌群多样性下降，菌群结构存在失衡，而高能量激光干预能够在一定程度上改善菌群紊乱，提高患者肠道菌群多样性。 |
| 英文摘要: |
| Objective: To study the structural characteristics of intestinal flora in patients with impaired glucose regulation (IGR) and explore the intervention effect of high energy laser. Methods: A total of 13 patients with IGR were included in the experimental group and 9 abdominal treatment sites were irradiated by 980nm semiconductor laser for one month (intervention group). At the same time, 10 healthy subjects were selected as the control group. Genomic DNA was extracted from the stool flora of the experimental group, intervention group and the control group for high-throughput sequencing. Results: At the phylum level, there was no significant difference in the relative abundance of Firmicutes, Bacteroidetes, Actinobacteria and Proteobacteria between the experimental group and control group (P>0.05). The relative abundance of Bacteroidetes in the intervention group was significantly higher than in the experimental group (P<0.05). At the level of genus, as compared with the control group, the relative abundance of Parabacteroides, Faecalibacterium in the experimental group decreased significantly (P<0.05). After laser intervention, the relative abundance of Parabacteroides increased significantly (P<0.05), while Streptococcus decreased significantly (P<0.05) as compared with that before intervention. In terms of species diversity, the diversity and evenness of the experimental group were significantly lower than those of the control group (P<0.05). As compared with the experimental group, the richness, diversity and evenness of the intervention group were significantly improved (P<0.05). Conclusion: The diversity of intestinal flora in patients with IGR is decreased and the structure of intestinal flora is unbalanced. High energy laser intervention can improve the disorder of intestinal flora to a certain extent and improve the diversity of intestinal flora in patients with IGR. |
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