| 卢苇,李娟,李晓东,杨梅,曹亚斌,吴锦秋.超声波调控Wnt3a/β-catenin通路对膝骨性关节炎大鼠软骨损伤的影响[J].中国康复,2026,41(4):195-201 |
| 超声波调控Wnt3a/β-catenin通路对膝骨性关节炎大鼠软骨损伤的影响 |
| Effect of ultrasonic regulation of Wnt3a/β-catenin pathway on cartilage injury in rats with knee osteoarthritis |
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| DOI：10.3870/zgkf.2026.04.001 |
| 中文关键词: 超声波 Wnt3a/β-catenin通路 膝骨性关节炎 软骨损伤 |
| 英文关键词: ultrasound Wnt3a/β-catenin pathway knee osteoarthritis cartilage injury |
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| 中文摘要: |
| 目的:探讨超声波调控Wnt3a/β-连环蛋白（Wnt3a/β-catenin）信号通路对膝骨性关节炎大鼠软骨损伤的影响。方法：通过右侧膝关节腔内注射碘乙酸钠（50μL）建立膝骨性关节炎大鼠模型，随机分为模型组、塞来昔布组、光疗组和超声波组，并以注射等剂量生理盐水为假手术组，各组样本量均为10只。塞来昔布组予以20mg/kg/d塞来昔布灌胃，光疗组予以威伐光照射20min/次，超声波组予以超声波治疗仪治疗10min/次，假手术组和模型组以生理盐水灌胃，均连续治疗20d。测量右膝关节活动度；采用HE染色和番红固绿染色法检测软骨细胞形态改变；采用酶联免疫吸附试验（ELISA）检测血清基质金属蛋白酶-13（MMP-13）、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和白细胞介素-1β（IL-1β）的表达水平；采用蛋白印迹技术（WB）检测软骨组织中Wnt3a、β-catenin、糖原合成酶激酶-3β（GSK-3β）和Runt相关转录因子2（Runx2）的相对表达水平；采用免疫组化技术（IHC）检测软骨组织中TNF-α、MMP-13的表达水平。结果：与假手术组比较，模型组、塞来昔布组、光疗组和超声波组右膝关节活动度减小（P＜0.05），血清MMP-13、TNF-α、IL-6、IL-1β水平升高（P＜0.05），软骨组织Wnt3a、β-catenin、Runx2、MMP-13和TNF-α表达水平升高（P＜0.05），软骨组织GSK-3β蛋白表达水平降低（P＜0.05）。与模型组比较，塞来昔布组、光疗组和超声波组右膝关节活动度增大（P＜0.05），血清MMP-13、TNF-α、IL-6、IL-1β水平、软骨组织Wnt3a、β-catenin、Runx2、MMP-13和TNF-α水平均降低（P＜0.05），软骨组织GSK-3β表达水平升高（P＜0.05）。与塞来昔布组和光疗组比较，超声波组右膝关节活动度增大（P＜0.05），血清MMP-13、TNF-α、IL-6、IL-1β水平、软骨组织Wnt3a、β-catenin、Runx2、MMP-13和TNF-α水平均降低（P＜0.05），软骨组织GSK-3β表达水平升高（P＜0.05）。塞来昔布组和光疗组各项指标比较差异无统计学意义。结论：超声波通过调控Wnt3a/β-catenin信号通路改善大鼠膝骨性关节炎软骨损伤。 |
| 英文摘要: |
| Objective: To investigate the effect of ultrasonic regulation of Wnt3a/β-catenin signaling pathway on cartilage injury in rats with knee osteoarthritis. Methods: The rat model of knee osteoarthritis was established by injecting sodium iodoacetate (50 μL) into the right knee joint cavity. The rats were randomly divided into model group, celecoxib group, phototherapy group and ultrasound group, and the same dose of normal saline was injected in the sham operation group. Each group included 10 subjects. Celecoxib group was given 20 mg/kg/d celecoxib by gavage, phototherapy group was given 20 min/time of light irradiation, ultrasound group was treated with ultrasonic therapy instrument for 10 min/time, sham operation group and model group were given normal saline by gavage, all of which were treated continuously for 20 days. The activity of the right knee joint was measured, the morphological changes of chondrocytes were observed by hematoxylin-eosin (HE) staining and safranin green staining. The expression levels of serum matrix metalloproteinase-13 (MMP-13), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) were detected by enzyme-linked immunosorbent assay (ELISA). The relative expression levels of Wnt family member 3a (Wnt3a), beta-catenin (β-catenin), glycogen synthase kinase-3β (GSK-3β) and Runt-related transcription factor 2 (Runx2) in cartilage tissue were detected by Western blotting (WB). The expression levels of TNF-α and MMP-13 in cartilage tissue were detected by immunohistochemistry (IHC). Results: Compared with the sham operation group, the range of motion of the right knee joint in the model group, the celecoxib group, the phototherapy group and the ultrasound group decreased, the levels of serum MMP-13, TNF-α, IL-6, IL-1β, the expression levels of Wnt3a, β-catenin, Runx2, MMP-13 and TNF-α in cartilage tissue increased (P<0.05), and the expression level of GSK-3β protein in cartilage tissue decreased (P<0.05). Compared with the model group, the right knee joint activity of the celecoxib group, the phototherapy group and the ultrasound group increased (P<0.05), the levels of serum MMP-13, TNF-α, IL-6, IL-1β, Wnt3a, β-catenin, Runx2, MMP-13 and TNF-α in cartilage tissue decreased (P<0.05), and the expression level of GSK-3β in cartilage tissue increased (P<0.05). Compared with the celecoxib group and the phototherapy group, the range of motion of the right knee joint of the ultrasound group increased (P<0.05), the levels of serum MMP-13, TNF-α, IL-6 and IL-1β, the levels of Wnt3a, β-catenin, Runx2, MMP-13 and TNF-α in cartilage tissue decreased (P<0.05), and the expression level of GSK-3β in cartilage tissue increased (P<0.05). There was no statistically significant difference in the above indicators between the celecoxib group and the phototherapy group. Conclusion: Ultrasound improves cartilage injury in rats with knee osteoarthritis by regulating Wnt3a/β-catenin signaling pathway. |
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