| Effect of S100A4 Protein on Neurite Outgrowth and Tubulin Polymerization in N2a Cells |
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| DOI： |
| EN KeyWords: S100A4 N2a cells tubulin |
| Fund Project:国家自然科学基金资助项目(30801220) |
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| EN Abstract: |
| Objective: To study the effects of S100A4 protein on neurite outgrowth and tubulin polymerization in N2a cells. Methods: N2a cells were cultured in vitro, and treated with 5 μmol/L S100A4 protein. Neurite outgrowth in N2a cells was examined morphologically at 0, 12, 24 and 36 h. The mount of polymerized tubulin was determined by Western blotting. Results: At 12th, 24th and 36th hour after the culture with 5 μmol/L S100A4 protein or DMSO, neurite outgrowth in N2a cells was observed. Neurite outgrowth was significantly greater in S100A4 protein-treated N2a cells than in DMSO-treated controls (P<0.05). The formation of neurite network was also observed in S100A4 protein group at 36th hour. Furthermore, 5 μmol/L S100A4 protein decreased the mount of depolymerizated tubulin compared to DMSO at 12th, 24th and 36th hour. The mount of GAPDH protein had no significant change in S100A4 protein group and DMSO group. Conclusion: The results suggest that 5 μmol/L S100A4 protein can improve neurite outgrowth through inhibiting microtubule depolymerization and promoting tubulin polymerization in vitro. |
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