| Effects and mechanism of repetitive transcranial magnetic stimulation (rTMS) on expression of PSD-95 and GAP-43 in hippocampus of rats with cerebral infarction |
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| DOI： |
| EN KeyWords: cerebral infarction rTMS PKA-CREB PSD-95 GAP-43 |
| Fund Project:教育部高等学校博士学科点专项科研基金（20090142110013），国家自然科学基金（81071601），国家自然科学基金（81171858） |
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| EN Abstract: |
| Objective:To investigate the effects of high-frequency repetitive transcranial magnetic stimulation (rTMS) on the expression of PSD-95 and GAP-43 and its mechanism in the ischemic hippocampus of rats with cerebral infarction.Methods:A total of 20 SD rats were divided into model control group(A),rTMS group(B),with 6 cases in every group,rTMS+NS group(C) and rTMS+H89 group(D),with 4 cases in every group.Reperfusion model with middle cerebral artery occlusion was established.rTMS of 20Hz was given to successful models for 7 days.Utrastructures of the ischemic CA1 and expression changes of PSD-95 and GAP-43 in rats of group A and B were investigated under the transmission electron microscopy and western blot respectively.The expression of pCREB,PSD-95 and GAP-43 were detected in the group C and D.Results:As compared with group A,the PSD-95 and GAP-43 expression levels were increased in group B(P<0.01).Under the transmission electron microscopy,the synaptic volume was increased,the anterior and posterior electron dense areas of the synapse were widened,the electron density and synaptic vesicles quantity were increased in group B.As compared with group C,the expression levels of pCREB,PSD-95and GAP-43 were decreased in group D(P<0.01).Conclusion:rTMS could promote PSD-95 and GAP-43 expression in the ischemic hippocampus after cerebral ischemia probably by regulating the PKA-CREB pathway. |
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