| Objective:To investigate the effect of scalp-point penetration needling on the expression of nestin in hippocampal dentate gyrus of rats with acute cerebral ischemia,and the mechanism for scalp-point penetration needling resisting the injury of acute cerebral ischemia.Methods:All 32 SD rats were randomly divided into four groups,with 8 rats in each group:normal group,sham-operated group,cerebral ischemia group and scalp-point penetration needling group.Focal cerebral ischemia model of rats was made by middle cerebral artery occlusion using a thread block technique.The rats in scalp-point penetration needling group were subjected to electro-acupuncture (EA) at Baihui (GV20) through Qianding (GV21) and Shuaigu (GB8) through Xuanli (GB6) with G6805-Ⅱ electro-acupuncture instrument after the rats woke up.After treatment,the neurologic deficit score was recorded and nestin in hippocampal deneate gyrus was detected by using immunohistochemistry.Results:After treatment for 7 days,the neurologic deficit score in scalp-point penetration needling group was obviously higher than in cerebral ischemia group (P<0.01).As compared with sham-operated group and normal control group,the expression of nestin in hippocampal dentate gyrus of scalp-point penetration needling group was increased greatly (P<0.01).As compared with cerebral ischemia group,the expression of nestin in scalp-point penetration needling group was significantly increased (P<0.01).Conclusion:The neurologic impairment of rats with cerebral ischemia was improved obviously and the expression of nestin in hippocampal dentate gyrus increased by scalp-point penetration needling,indicating that scalp-point penetration needling can resist cerebral ischemia injury. |
