| Objective:To investigate the expression and function of Cdh1-siRNA in cerebral ischemia-reperfusion injury of rats.Methods:All 150 male Sprague-Dawlcy rats were randomly divided into normal saline group (group A,n=50),lentivirus vector group (group B,n=50) and recombinant lentivirus group (group C,n=50).The rats in 3 groups were injected with normal saline, lentivirus vector and recombinant lentivirus respectively.At the 3rd day after injection,cerebral ischemia-reperfusion injury model of rat was established by modified four-vessel occlusion (4-VO) method.The expression of Cdh1 mRNA and Cyclin B was detected by quantitative real-time PCR and Western blotting.Apoptosis index (AI) was examined by using TUNEL staining method and the behavior was evaluated with Morris water maze test at the 7th day.Results:The expression of Cdh1 mRNA in group C was significantly lower than that in groups A and B (P<0.05),but that of Cyclin B and the levels of AI in group C were significantly higher than those in groups A and B (P<0.05).In addition,Morris water maze tests revealed that the latent period of seeking the platform in group C was longer than that in the other two groups from the 9th to 11th day after surgery.Conclusion:Cyclin B accumulation by APC-Cdh1 may mediate apoptosis of ischemic neurons. |
