| Objective:To observe the influence of electroacupuncture therapy on cortex regional cerebral blood flow (rCBF) and EPO expression of SD rats after cerebral ischemia reperfusion.Methods:Eighty SD rats were randomly divided into normal group (n=10),sham-operation group (n=10),model group (n=30) and electroacupuncture group (EA group) (n=30).The later two groups were further divided into 24h, 48h and 72h subgroups respectively,with 10 cases each.The model of focal cerebral ischemia reperfusion was established by inserting nylon thread into the middle cerebral artery occlusion (MCAO) and reperfusion except control group and sham-operation group in which the artery was separated only.EA group was treated with electroacupuncture therapy.The expression of EPO was detected by using immunohistochemistry and Western blotting.The rCBF was measured using a Doppler laser blood stream detector.Results:The expression of EPO in model group and EA group was increased at 24 h,peaked at 48 h,and decreased at 72 h,and that in EA group was significantly increased as compared with that in in model group at the 3 time points (P<0.05 or 0.01).The rCBF in model group and EA group was consecutively increased at the three time points,and that in EA group significantly increased as compared with that in model group at the 3 time points (P<0.05 or 0.01).Conclusion:Electroacupuncture in rats can promote the expression of EPO and increase the rCBF in ischemic brain tissue after focal cerebral ischemia reperfusion,which may be helpful to alleviate the damage of brain tissue. |
