| Objective:To investigate the influence of electroacupuncture and magnetic stimulation therapy on the detrusor excitability and α-SMA of rats with spinal cord injury (SCI) and the mechanism.Methods:Forty SD rats were randomly divided into 5 groups:normal group,model group,electroacupuncture group,magnetic stimulation group,and combination group.The SCI model was prepared by heavy objects fall method.The minimum shrinkage tension and contraction frequency of isolated detrusor muscle strips in rats were measured.Immunohistochemical methods were adopted to quantify the α-SMA positive cells of the detrusor.Results:After 10 times of treatment,the minimum shrinkage tension in combination group was obviously lower than model group,electroacupuncture group and magnetic stimulation group,and that in electroacupuncture group and magnetic stimulation group was lower than in model group (all P<0.05).The contraction frequency and the number of α-SMA positive cells in combination group were obviously increased as compared with model group,electroacupuncture group and magnetic stimulation group,and those in electroacupuncture group and magnetic stimulation group were significantly increased as compared with model group (all P<0.05).Conclusion:Electroacupuncture and magnetic stimulation therapy could significantly increase α-SMA content of the detrusor and detrusor excitability,and improve urinary retention condition,and the combination of electroacupuncture with magnetic stimulation treatment showed more satisfactory effectiveness. |
