| A focal brain trauma model for rehabilitation study |
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| DOI： |
| EN KeyWords: traumatic brain injury controlled cortical impact animal model rehabilitation |
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| 作者 | 单位 | | 沈夏锋 | 上海市第一康复医院（筹）,上海杨浦区老年医院康复医学科,上海 200090 | | 吴军发 | 复旦大学附属华山医院康复医学科,上海 200040 | | 于惠贤 | 首都医科大学附属北京同仁医院康复医学科,北京 100730 | | 张宇玲 | 上海理工大学医疗器械与食品学院,上海 200093 | | 田闪 | 复旦大学附属华山医院康复医学科,上海 200040 | | 路微波 | 上海市第一康复医院（筹）,上海杨浦区老年医院康复医学科,上海 200090 | | 胡永善 | 复旦大学附属华山医院康复医学科,上海 200040 | | 吴毅 | 复旦大学附属华山医院康复医学科,上海 200040 |
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| EN Abstract: |
| Objective:To establish an animal experimental focal traumatic brain injury (TBI) model for investigating the underlying mechanisms of rehabilitation.Methods:After adult SD rats had received a severe controlled cortical impact (CCI) injury,they were randomly assigned to TBI group (n=8) or sham operation group (n=8).After TBI,the neurological deficits scoring,foot-fault test and cylinder test were performed.On 21-25 days post-injury,rats were tested for spatial learning and memory in a Morris Water Maze.On 28 days post-injury,animals were sacrificed and evaluated for quantitative cortical lesion volume and pathological changes.Results:The rats in TBI group demonstrated declined neurological deficits scores,coordination and asymmetry compared to sham operation group (P<0.05).The rats in TBI group showed obvious deficits in spatial learning and memory (P<0.05).We also observed significant change in lesion volume,neuron loss and overexpression of microglia around the lesion in TBI group (P<0.05).Conclusion:Rats undergoing a severe CCI injury showed significant sensorimotor,cognitive performance impairment and pathological changes.It is suggest that the model should be useful for assessing the effects of rehabilitation in TBI. |
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