| Objective:To explore the effects of bone marrow mononuclear cells (BMMNCs) on the expression of neurotrophin-3 (NT-3) and synaptophysin (SYN) in the injured spinal cord of rats with complete transection of the spinal cord (SCT).Methods:The rat model of complete SCT was set up,and 146 rats were divided randomly into Sham group (n=48),SCT model group (n=49) and experimental group (n=49).Sham group,removing the corresponding lamin and suturing muscle and skin separately;SCT model group,establishing SCT models;experimental group,given BMMNCs transplantation in the SCT models.On the postoperative day (POD) 5,7,14 and 21,the rat hindlimb was scored by the scale of Basso,Beattie and Bresnahan (BBB Grading),and the expression levels of NT-3 and SYN were detected by the immunohistochemistry and RT-PCR.Results:On the POD 5,7,14 and 21,BBB scores in experimental group and SCT model group were significantly lower than in Sham group (P<0.05),and those in SCT model group were lower than in experimental group (P<0.05).RT-PCR and immunohistochemistry revealed the expression levels of NT-3 mRNA and number of NT-3 positive cells in experimental group and SCT model group were significantly increased as compared with Sham group (P<0.05),and those in SCT group were higher than in experimental group (P<0.05).The positive particles of SYN and SYN mRNA in experimental group and SCT model group were significantly increased as compared with Sham group (P<0.05),and those in SCT model group were increased as compared with those in experimental group (P<0.05).Conclusions:BMMNCs can promote the expression of NT-3 and SYN in injured spinal cord tissues,which may be one of the mechanisms by which BMMNCs repair spinal cord injury. |
