| Effect of acute carbon monoxide poisoning on oligodendrocyte precursor cells in rats |
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| DOI： |
| EN KeyWords: acute carbon monoxide poisoning demyelination myelin basic protein chondroitin sulfate proteoglycan NG2 oligodendrocyte precursor cells |
| Fund Project:海军总医院创新培育基金资助（CXPY201303） |
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| EN Abstract: |
| Objective: To observe the changes in myelin and expression of myelin basic protein (MBP) and NG2 in the brain tissue of rats through the establishment of the acute carbon monoxide poisoning (ACOP) model, and to provide experimental evidence for the mechanism of demyelination of the central nervous system after ACOP. Methods: Twenty-four male rats were divided into the control group (n=12) and the intoxication group (n=12) randomly. Animals in the intoxication group were intraperitoneally injected with pure CO repeatedly, and those in the control group with air by the same protocol. The brain tissues were taken from the animals of the two groups at 1st and 3rd day after the establishment of the model. Changes in myelin were observed by immunohistochemical staining, and the expression of MBP and NG2 was detected using immunohistochemistry and Western blot. Results: Immunohistochemistry demonstrated that the expression levels of MBP and NG2 began to decrease at 1st day after ACOP, and there was statistically significant difference in the integral optical density (IOD) level of MBP and NG2 as compared with the control group at 3rd day after ACOP (P<0.05). Results of Western blot showed that as compared with the control group, the expression levels of MBP and NG2 were decreased at 1st day after ACOP, and decreased further at 3rd day (P<0.05). Conclusion: Demyelination and damage of oligodendrocyte precursor cells occurred in the brain tissue of rats after ACOP. |
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