| Objective:To explore the heat production mechanism of diet-induced obese (DIO) rats after 8-week aerobic exercise by observing the expression of BMP7, AMPK and COX2 in the hypothalamus and brown adipose tissue. Methods:The model of DIO SD male rats was established. DIO exercise group exerted 8-week aerobic exercise. BMP7 and COX2 protein expression was detected by Western blotting. Adipose/body mass, body mass, RMR, and NST were detected too. Results: BMP7 protein expression in the hypothalamus and brown adipose tissue was significantly increased in DIO exercise group as compared with obesity quiet group. As well as, COX2 protein expression was increased in DIO exercise group. Adipose/body mass, body mass, RMR, and NST in DIO exercise group were significantly decreased as compared with obesity quiet group. Conclusions: Eight-week aerobic exercise could facilitate brown adipose tissue heat production by increasing BMP7 protein expression in the hypothalamus and brown adipose tissue of DIO rats and adjusting energy metabolism function. |
