| Effects of LINGO-1 on oligodendrocyte differentiation of spinal cord-derived neural stem cells |
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| DOI： |
| EN KeyWords: LINGO-1 spinal cord-derived neural stem cells oligodendrocyte differentiation |
| Fund Project:国家自然科学基金（81100932）；中国博士后科研基金（20100481518）；科技部国际合作重点专项基金（2013DFA32610） |
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| EN Abstract: |
| Objective: To investigate the expression of LINGO-1 in the oligodendrocyte differentiation of spinal cord-derived neural stem cells (SpNSCs). Methods: SpNSCs were isolated from rat spinal cord and cultured in vitro. The expression of LINGO-1 was detected by double immunofluorescence staining of LINGO-1 and A2B5, O4 at 3th and 5th day of differentiation. SpNSCs isolated from rat spinal cord were cultured in vitro and divided into RNAi group and control group. The RNAi group was transfected with LINGO-1 shRNA lentiviral vector to down-regulate the expression of LINGO-1, and control group was transfected with lentiviral vector. The oligodendrocyte was examined by immunofluorescence staining and MBP expression was detected using Western blotting at 7th day of differentiation. Results: LINGO-1 was co-expressed with A2B5 and O4. After down-regulation of LINGO-1 expression, the number of mature oligodendrocytes was 3.28 fold over the control group, and the expression of MBP was 5.31 fold over the control group (P<0.05 for all). Conclusion: LINGO-1 was constantly expressed and had a negative effect on the differentiation of oligodendrocytes. |
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