| Objective: To investigate the optimal conditions of inhibiting the β-catenin expression in neural stem cells (NSCs) for harvesting NSCs low-expressing β-catenin, and exploring the roles of Wnt/β-catenin pathway in proliferation of NSCs induced by the high-intensity pulsed electromagnetic fields. Methods: (1) NSCs were derived from subventricular zone (SVZ) of newborn SD rats, and then cultured for 14 days using serum-free medium. (2) The RNAi method was used to inhibit β-catenin expression in NSCs, and the optimal MOI for transfection to inhibit β-catenin levels in NSCs was measured. (3) The NSCs were divided into the negative control group and experimental group, and then they were exposed in the high-intensity pulsed electromagnetic fields with stimulus condition of 4T, 0.1Hz, 8 times. (4) At 1st and 7th day after the intervention with the high-intensity pulsed electromagnetic fields, the proliferation of NSCs was tested by the CCK8 assay. Results: (1) When MOI=10, the best inhibitory effects on β-catenin could be obtained in NSCs. (2) At 1st and 7th day after NSCs were exposed to the high-intensity pulsed electromagnetic fields, the proliferation of NSCs in the negative control group was significantly higher than in the experimental group (P<0.05). Conclusion: The Wnt/β-catenin pathway may play a role in proliferation of NSCs induced by the high-intensity pulsed electromagnetic fields. |
