| Effects of electroacupuncture at Quchi and Zusanli on microglia and exosome protein in ischemic motor cortex of ischemia reperfusion rats and mechansim |
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| DOI： |
| EN KeyWords: cerebral ischemia M2 type of microglia exosomes electroacupuncture |
| Fund Project:国家自然科学基金（81373778）；福建省自然科学基金项目（2016J01397） |
| 作者 | 单位 | | 金婷婷 | 1．福建中医药大学康复医学院,福州 350122；2.福建省康复技术重点实验室,福州 350122 | | 柳维林 | 福建中医药大学康复医学院,福州 350122；福建康复产业研究院技术创新平台,福州 350122 | | 李钻芳 | 福建中西医结合研究院,福州 350122 | | 李建鸿 | 福建中医药大学康复医学院,福州 350122；福建康复产业研究院技术创新平台,福州 350122 | | 张嘉泳 | 福建中医药大学康复医学院,福州 350122 | | 李乐 | 福建中医药大学康复医学院,福州 350122 | | 张宇豪 | 福建中医药大学康复医学院,福州 350122 | | 陈立典 | 福建中医药大学康复医学院,福州 350122；国家中医药管理局中医康复研究中心,福州 350122 | | 陶静 | 福建中医药大学康复医学院,福州 350122；国家中医药管理局中医康复研究中心,福州 350122 |
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| EN Abstract: |
| Objective：To investigate the expression of M2 microglia-derived exosomes in the ischemic motor cortex of rats with ischemia reperfusion injury, and to observe the effects of electroacupuncture at Quchi and Zusanli on motor function in rats with ischemia reperfusion injury and possible mechanisms. Methods: Thirty-six male Sprague-Dawley rats were randomly divided into sham operation group, model group and electroacupuncture group (n=14 each). Zea-Longa neurobehavioral score was used for neurological deficit assessment. Catwalk treadmill experiment was used to evaluate motor function. The number of M2 microglia positive cells in the ischemic motor cortex was marked by immunohistochemistry, and the expression of exosome protein CD81 was detected by immunofluorescence double labeling. Results: After 7 days of electroacupuncture intervention, the Zea Longa score was significantly reduced (P<0.05), the walking speed increased significantly (P<0.01), the duration decreased significantly (P<0.05), the paw print area of the rats increased significantly (P<0.001), and the number of M2 type microglia and the secretion of exogenous protein CD81 increased significantly in the ischemic motor cortex (P<0.01) in the electroacupuncture group as compared with those in the model group. Conclusion: Electroacupuncture at Quchi and Zusanli can promote the secretion of M2 type of microglia exosomes in the ischemic motor cortex, play a neuroprotective role and effectively improve the motor function of rats with ischemia-reperfusion injury. |
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