| Objective：To investigate the expression of M2 microglia-derived exosomes in the ischemic motor cortex of rats with ischemia reperfusion injury, and to observe the effects of electroacupuncture at Quchi and Zusanli on motor function in rats with ischemia reperfusion injury and possible mechanisms. Methods: Thirty-six male Sprague-Dawley rats were randomly divided into sham operation group, model group and electroacupuncture group (n=14 each). Zea-Longa neurobehavioral score was used for neurological deficit assessment. Catwalk treadmill experiment was used to evaluate motor function. The number of M2 microglia positive cells in the ischemic motor cortex was marked by immunohistochemistry, and the expression of exosome protein CD81 was detected by immunofluorescence double labeling. Results: After 7 days of electroacupuncture intervention, the Zea Longa score was significantly reduced (P<0.05), the walking speed increased significantly (P<0.01), the duration decreased significantly (P<0.05), the paw print area of the rats increased significantly (P<0.001), and the number of M2 type microglia and the secretion of exogenous protein CD81 increased significantly in the ischemic motor cortex (P<0.01) in the electroacupuncture group as compared with those in the model group. Conclusion: Electroacupuncture at Quchi and Zusanli can promote the secretion of M2 type of microglia exosomes in the ischemic motor cortex, play a neuroprotective role and effectively improve the motor function of rats with ischemia-reperfusion injury. |
