| Effects of hyperbaric oxygen on expression of gap junctional protein Cx37 in brain and blood brain barrier after cerebral ischemia-reperfusion in rats |
| |
| DOI： |
| EN KeyWords: hyperbaric oxygen blood brain barrier ischemia-reperfusion Connexin37 |
| Fund Project: |
|
| View Counts: 9762 |
| PDF Download Counts: 6614 |
| EN Abstract: |
| Objective: To investigate the effects of hyperbaric oxygen (HBO) on the expression of gap junctional protein Cx37 in the brain and blood brain barrier (BBB) after cerebral ischemia reperfusion (CIR) in rats. Methods: 240 male Wistar rats were randomly assigned into sham operation, model, model+HBO, and HBO groups (n=60 each). Using CIR models, 0.25 MPa HBO was applied 5 times during the reperfusion period, and 2% Evens blue (EB) was injected into tail veins 1 h before the animals were executed. The expression of Cx37 mRNA and protein, and the contents of EB were determined by RT-PCR, Western blotting and spectrophotometer, respectively. Results: In model group, the EB content in brain tissue was significantly increased as compared with the sham operation group, reaching its peak at 4 h post CIR injury (P<0.01). The expression of Cx37 mRNA and protein significantly increased consecutively in different model group as compared with the 0 h group, reaching its highest level at 24 h. There was no significant difference in the expression of Cx37 mRNA and protein, and the content of EB between HBO group and sham operation group (P>0.05). The expression of Cx37 mRNA and protein in model+HBO group at CIR 24 h was significantly decreased as compared with model group (P<0.01). Conclusion: HBO intervention can decrease the permeability of BBB via reducing the expression level of Cx37 mRNA and protein. |
|
查看全文
Download PDF Reader HTML Full Text |
| Close |
|
|
|
