| Objective: To observe the effect of hyperbaric oxygen (HBO) on survival of human glioma 172 cells. Methods: Cultured human glioma 172 cells were randomly divided into control group and HBO group. The cells in control group were placed in chamber of 0.1 MPa for 90 min per day, and then cultured in 37℃ 5% CO2 incubator. The cells in HBO group were exposed to HBO with pressure increasing from 0.1 MPa to 0.2 MPa within 15 min, and maintaining at 0.2 MPa for 1 h, then decreasing to 0.1 MPa within 15 min per day, and then cultured in 37℃ 5% CO2 incubator. The cells were exposed to HBO 10 times. On the 11th day, cells of both groups were collected. Cell growth was observed under light microscopy, and apoptosis and cell growth cycle were detected by flow cytometry. The ultrastructure of cells was observed by electron microscopy. Results: The cell count of the HBO group was significantly less than that of the control group (P=0.01), and the proportion of early and late apoptotic cells in the HBO group was significantly higher than that in the control group (both P<0.01). There was no statistically significant difference in the proportion of G1 and S phase cells between the two groups. The proportion of G2 phase cells in the HBO group was significantly reduced as compared with the control group ( P<0.01). The protrusions of 172 cells in HBO group were shorter and smaller than those of control group. Conclusion: HBO can delay the growth of human glioma 172 cells, and promote apoptosis of 172 cells, with more cells blocking in the S phase, together with the protrusions shorten and reduced which related to tumor metastasis and recurrence, implying HBO has an inhibitory effect on human glioma 172 cells. |
