| Effects of Electroacupuncture Stimulation of “Jiaji(EX-B2)” on Expression of Caspase-3 and Caspase-9 in Rabbit Degenerated Intervertebral Disc |
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| DOI： |
| EN KeyWords: electroacupuncture jiaji(EX-B2): intervertebral disc degeneration caspase-3 caspase-9 |
| Fund Project:国家自然科学基金面上项目（No.81574072；No.81774392） |
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| EN Abstract: |
| Objective: To observe the effect of electroacupunture at jiaji(EX B2) on the expression of caspase 3 and caspase 9 in rabbit degenerated intervertebral disc. Method: A total of 20 New Zealand rabbits were randomly divided into normal, sham operation, model, and EA groups (n=5 each). Rabbits in normal group were given natural breeding without any treatment. The rabbits in model group were given the custom made external device on the bases of two K wires inserted into the vertebral bodies L4 and L5 parallel to the adjacent study for 28 days. There were nothing else treatment in this group. The rabbits in sham operation group were attached to two K wires inserted into the vertebral bodies L4 and L5 parallel to the adjacent study without any pressure loading devices or other treatment. The rabbits in EA group were given EA therapy from the first day of the disc degeneration model established. The expression levels of caspase 3 and caspase 9 in the lumbar intervertebral disc (L4 L5) tissues were assayed by immunohistochemistry at 28th day. Results: No significant changes were found between the control and sham groups at any time point for the expression of caspase 3 and caspase 9. Compared to the normal group, caspase 3 and caspase 9 expression levels at 28th day in the model group were apparently increased (all P<0.05). As compared with the model group, the expression levels of caspase 3 ancaspase 9 were remarkably decreased in the EA group at day 28 (P<0.05). Conclusion: The treatment of EA at jiaji(EX B2) has a protective effect on the tissues of the lumbar intervertebral disc in rabbits. Its mechanism of delaying the degeneration of the lumbar intervertebral disc may be related to the down regulation of caspase 3 and caspase 9. |
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