| Objective: To explore the effect of hyperbaric oxygen combined with early rehabilitation training on the expression of autophagy related protein Beclin-1 in hippocampus of rats with intracerebral hemorrhage and their behaviors. Methods：A total of 55 adult male SD rats were randomly divided into blank group, non-intervention model group, hyperbaric oxygen group, rehabilitation training group and combined intervention group, 10 rats in each group, and 5 rats were used for standby. Rats in non-intervention model group, hyperbaric oxygen group, rehabilitation training group and combined intervention group were injected with collagenase Ⅶ to establish cerebral hemorrhage model. Hyperbaric oxygen group and rehabilitation training group were given hyperbaric oxygen and rehabilitation training respectively on the 3rd day after operation, and the combined intervention group was subjected to the above interventions. On the 7th and 14th days after the intervention, 5 rats from each group were randomly selected and scored by neurobehavioral Longa score. After scoring, Beclin-1 was detected in the hippocampus by immunohisto-chemical method. Results: Compared with the non-intervention model group, the Longa scores of rats in hyperbaric oxygen group, rehabilitation training group and combined intervention group were significantly decreased (all P<0.05), and that in the combined intervention group was lower than the hyperbaric oxygen group and rehabilitation training group (both P<0.05). As compared with the non-intervention model group, Beclin-1 positive reaction in hippocampus of rats in hyperbaric oxygen group, rehabilitation training group and combined intervention group was significantly decreased (all P<0.05). The decrease amplitude of combined intervention group was greater than that of hyperbaric oxygen group and rehabilitation training group (both P<0.05). Conclusion: Hyperbaric oxygen and early rehabilitation training can effectively regulate the expression of autophagy related proteins in brain cells in the early stage of cerebral hem-orrhage, and hyperbaric oxygen combined with rehabilitation training is superior to hyperbaric oxygen and rehabilitation training alone. The improvement of neurobehavior after intracerebral hemorrhage may be related to the regulation of autophagy-related proteins in the brain. |
