| Objective:To explore the therapeutic effect of MSCs-derived exosomes (MSc-exo) on mouse Parkinson's disease（PD） model, so as to provide experimental basis for the treatment of PD and the application of MSC-exo. Methods: MSC-exo were extracted by ultracentrifugation, and identified by immunoblotting and transmission electron microscopy. C57BL/6 mice were divided into three groups: sham group, PD group and PD + MSC-exo group. C57BL/6 mice were injected intraperitoneally with MPTP to induce acute PD model. MSC-exo or PBS were injected into caudal vein. The behavioral changes of mice were analyzed by rotation experiment on the 4th, 14th and 30th days. Nissl staining and immunoblotting of Nrf2, HO1, TNFα and IL-1β were used to observe the effect of MSC-exo on PD model. Results: At 4th, 14th and 30th day, the number of turns in PD + MSc exo group was significantly less than that in PD group (P<0.0001). Compared with sham group, Nissl positive cells in PD group decreased significantly, while Nissl cells in PD + MSC-exo group increased significantly as compared with PD group. Western blot showed that as compared with sham group, TH expression in PD group decreased significantly, and Nrf2, keap1, TNFα and IL-1β increased significantly. The expression of TH increased significantly in PD + MSC-exo, but that of Nrf2, keap1, TNFα and IL-1β decreased significantly as compared with PD group. Conclusion: MSC-exo can protect neurons, decrease the loss of neurons in PD model and reduce the occurrence of inflammation. So, MSC-Exo have a satisfactory therapeutic effect on PD model. |
