| Objective: To study the structural characteristics of intestinal flora in patients with impaired glucose regulation (IGR) and explore the intervention effect of high energy laser. Methods: A total of 13 patients with IGR were included in the experimental group and 9 abdominal treatment sites were irradiated by 980nm semiconductor laser for one month (intervention group). At the same time, 10 healthy subjects were selected as the control group. Genomic DNA was extracted from the stool flora of the experimental group, intervention group and the control group for high-throughput sequencing. Results: At the phylum level, there was no significant difference in the relative abundance of Firmicutes, Bacteroidetes, Actinobacteria and Proteobacteria between the experimental group and control group (P>0.05). The relative abundance of Bacteroidetes in the intervention group was significantly higher than in the experimental group (P<0.05). At the level of genus, as compared with the control group, the relative abundance of Parabacteroides, Faecalibacterium in the experimental group decreased significantly (P<0.05). After laser intervention, the relative abundance of Parabacteroides increased significantly (P<0.05), while Streptococcus decreased significantly (P<0.05) as compared with that before intervention. In terms of species diversity, the diversity and evenness of the experimental group were significantly lower than those of the control group (P<0.05). As compared with the experimental group, the richness, diversity and evenness of the intervention group were significantly improved (P<0.05). Conclusion: The diversity of intestinal flora in patients with IGR is decreased and the structure of intestinal flora is unbalanced. High energy laser intervention can improve the disorder of intestinal flora to a certain extent and improve the diversity of intestinal flora in patients with IGR. |
