文章摘要
李建鸿,黄佳,宋长明,杨敏光,金婷婷,柳维林,陶静,陈立典.缺血再灌注损伤大鼠学习记忆能力损伤及其与海马突触素表达水平的相关性研究[J].中国康复,2019,34(2):59-63
缺血再灌注损伤大鼠学习记忆能力损伤及其与海马突触素表达水平的相关性研究
Relationship between learning-memory function impairment and synaptophysin expression in hippocampus of rats with ischemia reperfusion injury
  
DOI:
中文关键词: 脑缺血再灌注  学习记忆  海马  突触素
英文关键词: Ischemia-reperfusion  Learning-memory  Hippocampus  Synaptophysin
基金项目:国家自然科学基金资助项目(81403462);福建省教育厅资助省属高校项目(JK2014022)
作者单位
李建鸿 福建中医药大学康复医学院福建省康复技术重点实验室福建康复产业研究院技术创新平台国家中医药管理局中医康复研究中心福州 350122 
黄佳 福建中医药大学康复医学院福建省康复技术重点实验室福建康复产业研究院技术创新平台国家中医药管理局中医康复研究中心福州 350122 
宋长明 福建中医药大学康复医学院福建省康复技术重点实验室福建康复产业研究院技术创新平台国家中医药管理局中医康复研究中心福州 350122 
杨敏光 福建中医药大学康复医学院福建省康复技术重点实验室福建康复产业研究院技术创新平台国家中医药管理局中医康复研究中心福州 350122 
金婷婷 福建中医药大学康复医学院福建省康复技术重点实验室福建康复产业研究院技术创新平台国家中医药管理局中医康复研究中心福州 350122 
柳维林 福建中医药大学康复医学院福建省康复技术重点实验室福建康复产业研究院技术创新平台国家中医药管理局中医康复研究中心福州 350122 
陶静 福建中医药大学康复医学院福建省康复技术重点实验室福建康复产业研究院技术创新平台国家中医药管理局中医康复研究中心福州 350122 
陈立典 福建中医药大学康复医学院福建省康复技术重点实验室福建康复产业研究院技术创新平台国家中医药管理局中医康复研究中心福州 350122 
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中文摘要:
  目的:观察缺血再灌注损伤大鼠学习记忆能力损伤及其与海马突触素(SYN)蛋白表达的相关性。方法:将于SPF饲养的雄性SD大鼠16只分为模型组10只和对照组6只。模型组大鼠采用改良Longa线栓阻塞法制备左侧大脑中动脉梗塞(middle cerebral artery occlusion, MCAO)模型,将符合纳入标准的6只模型组大鼠和6只对照组大鼠纳入实验。Zea Longa神经行为学评分法检测大鼠的神经功能缺损状况;T2加权像(T2 weighted image,T2WI)扫描观察大鼠脑梗死体积;巴恩斯迷宫检测大鼠的学习记忆能力;蛋白免疫印迹法检测大鼠海马突触素SYN的表达水平。将模型组大鼠SYN表达水平与逃避潜伏期、进入错误洞口次数做相关性分析。结果:造模2h后,模型组大鼠Zea Longa评分升高;造模24h后,T2加权像显示模型组大鼠出现脑梗死;巴恩斯迷宫数据显示与对照组相比,模型组大鼠找到正确洞口的时间明显延长(P<0.01),其进入错误洞口次数显著明显增多(P<0.01);蛋白免疫印迹检测结果显示与对照组相比,模型组大鼠海马SYN表达量显著下降(P<0.01)。相关性统计结果显示模型组大鼠SYN表达水平与大鼠逃避潜伏期以及进入错误洞口次数呈显著负相关(P<0.05):SYN表达水平与大鼠逃避潜伏期相关系数r=-0.916(P<0.05);SYN表达水平与大鼠进入错误洞口次数相关系数r=-0.87(P<0.05)。结论:脑缺血再灌注损伤大鼠学习记忆能力下降可能与海马突触素SYN表达减少,从而使突触可塑性受损有关,并且SYN下降越明显,大鼠学习记忆能力损伤越严重。
英文摘要:
  Objective:To observe the impairment of learning and memory ability and its correlation with synaptophysin (SYN) protein expression in hippocampus of rats with ischemia-reperfusion injury. Methods: Sixteen male Sprague-Dawley (SD) rats raised in SPF were randomly divided into model (n=10) and control (n=6) groups. Rats in the model group were treated with the modified Longa' methods to create the left middle cerebral artery occlusion (MCAO) model. Rats of the model (n=6) and the control (n=6) groups that met the inclusion criteria were included. Neurological deficit was assessed by Zea Longa neurobehavioral scores tests. T2 weighted image (T2WI) scan was used to observe the volume of cerebral infarction in rats. The function of learning and memory was tested by Barnes maze. The SYN expression in the hippocampus was detected by Western blotting. The correlation between the expression level of SYN in the model group and the escape latency of rats and the number of entrance into the wrong holes was analyzed. Results: After 2 h of operation, the Zea-Longa scores in the model group increased and the symptoms of neurological deficits appeared; After 24 h of operation, T2WI scan showed that the model group had cerebral infarction. As compared with the control group, the Barnes data showed that the escape latency in the model group was significantly prolonged (P<0.01), and the number of times into the wrong holes was significantly increased (P<0.01); the expression of SYN in the hippocampus of the model group was significantly decreased (P<0.01). Correlation analysis showed that the expression level of SYN was significantly negatively correlated with the escape latency for a correct hole and the number of times into the wrong holes. The correlation coefficient between SYN expression level and the escape latency of rats was r=-0.916 (P<0.05); the correlation coefficient between SYN expression level and the number of rats entering the wrong entrance holes was r=-0.87 (P<0.05). Conclusion: The impairment of learning and memory function in rats with cerebral ischemia-reperfusion injury may be related to the decrease of SYN expression in the hippocampus, which may result in the damage of synaptic plasticity.
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