| 罗静,邓石峰,余雨荷,刘笑萌,艾坤,许明,张泓.完全性骶髓损伤后大鼠神经源性膀胱模型的制备与观察[J].中国康复,2022,37(7):387-391 |
| 完全性骶髓损伤后大鼠神经源性膀胱模型的制备与观察 |
| Establishment and application of neurogenic bladder model after complete sacral cord injury in rats |
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| DOI： |
| 中文关键词: 骶髓损伤 神经源性膀胱 尿流动力学 动物模型 |
| 英文关键词: sacral cord injury neurogenic bladder urodynamics animal model |
| 基金项目:国家自然科学基金面上项目(81874510)；湖南省高等学校“国内一流培育学科”中西医结合学科经费资助（NO.2020ZXYJH23）；长沙市自然科学基金项目（NO. KQ2007041） |
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| 中文摘要: |
| 目的：对完全性骶髓损伤后大鼠神经源性膀胱模型（NB）进行制备并观察验证，以期为骶髓损伤后神经源性膀胱模型大鼠的制备提供实验依据。方法：42只健康雌性SD大鼠随机抽取12只设为空白组，常规饲养后于第19天行尿流动力学检测。剩余30只大鼠采用改良Hassan Shaker脊髓横断法制成骶髓损伤大鼠NB模型，成模后随机分为3个次组（A/B/C组），每组10只；模型组大鼠造模术后行规范化护理，观察记录大鼠膀胱功能、手法排尿量及并发症等情况；A/B/C组大鼠分别于造模术后第19、23、28天行尿流动力学检测，检测结束后断颈处死大鼠，膀胱逼尿肌苏木素-伊红染色（HE）后后镜下观察其形态变化。结果：模型组大鼠造模术后28天内膀胱功能障碍持续表现为尿潴留；术后第19天模型组大鼠生命体征稳定，并发症控制良好；与空白组进行对比：光镜下可见模型组大鼠逼尿肌肌束排列紊乱、肌纤维萎缩严重、间质结构填充增多等病理性改变；尿流动力学检测结果显示：与空白组相比较，模型组大鼠膀胱漏尿点压力(LPP)降低、膀胱最大容量(MCC)、膀胱顺应性(BC)增加，差异均具有统计学意义（P＜0.01）。A/B/C组大鼠造模术后第19、23、28天尿流动力学检测结果比较显示：3个阶段大鼠膀胱漏尿点压力、最大容量、顺应性无统计学差异。结论：于大鼠L2-3节段采用改良的Hassan Shaker法完全横断SD大鼠骶段脊髓，成功复制完全性骶髓损伤大鼠神经源性膀胱模型，且模型质量稳定可靠。 |
| 英文摘要: |
| Objective: To establish and verify the neurogenic bladder (NB) model of rats after complete sacral cord injury. Methods: A total of 12 rats were randomly selected from 42 female SD rats as blank group, and urodynamic test was performed at the 19th day after conventional feeding. The remaining rats were used to establish the NB model of sacral SCI by modified Hassan shaker spinal cord transection, and randomly divided into three experimental groups: groups A, B and C, with 10 rats in each group after modeling. After modeling, the rats in the model group were given standardized nursing care. The bladder function, manual micturition and complications were observed and recorded. At the 19th, 23rd and 28th day after modeling, the rats in the groups A, B and C were tested for urodynamic measurements. Results: Bladder dysfunction in model group was urinary retention within 28 days after modeling; At the 19th day after operation, the vital signs of rats in the model group were stable and the complications were well controlled. As compared with the blank group, pathological changes such as disordered arrangement of detrusor muscle bundles, serious atrophy of muscle fibers and increase of interstitial structure filling were observed in the model group under the light microscope. Urodynamic examination showed that the LPP decreased, the MCC and BC of bladder increased (P<0.01). The results of urodynamics in the model group were compared withthose at the 19th, 23rd and 28th day after modeling, and there were no significant changes in the LPP, MCC and BC of bladder in the three stages, suggesting that dysfunction of urinary retention persisted in rats of model group. Conclusion: Using the improved Hassan shaker method to completely transect the L2-3 spinal cord of SD rats successfully reproduced the NB model of rats, and the quality of the model was stable and rel-iable. |
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