| Relationship between learning-memory function impairment and synaptophysin expression in hippocampus of rats with ischemia reperfusion injury |
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| DOI： |
| EN KeyWords: Ischemia-reperfusion Learning-memory Hippocampus Synaptophysin |
| Fund Project:国家自然科学基金资助项目(81403462)；福建省教育厅资助省属高校项目(JK2014022) |
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| EN Abstract: |
| Objective：To observe the impairment of learning and memory ability and its correlation with synaptophysin (SYN) protein expression in hippocampus of rats with ischemia-reperfusion injury. Methods: Sixteen male Sprague-Dawley (SD) rats raised in SPF were randomly divided into model (n=10) and control (n=6) groups. Rats in the model group were treated with the modified Longa' methods to create the left middle cerebral artery occlusion (MCAO) model. Rats of the model (n=6) and the control (n=6) groups that met the inclusion criteria were included. Neurological deficit was assessed by Zea Longa neurobehavioral scores tests. T2 weighted image (T2WI) scan was used to observe the volume of cerebral infarction in rats. The function of learning and memory was tested by Barnes maze. The SYN expression in the hippocampus was detected by Western blotting. The correlation between the expression level of SYN in the model group and the escape latency of rats and the number of entrance into the wrong holes was analyzed. Results: After 2 h of operation, the Zea-Longa scores in the model group increased and the symptoms of neurological deficits appeared; After 24 h of operation, T2WI scan showed that the model group had cerebral infarction. As compared with the control group, the Barnes data showed that the escape latency in the model group was significantly prolonged (P<0.01), and the number of times into the wrong holes was significantly increased (P<0.01); the expression of SYN in the hippocampus of the model group was significantly decreased (P<0.01). Correlation analysis showed that the expression level of SYN was significantly negatively correlated with the escape latency for a correct hole and the number of times into the wrong holes. The correlation coefficient between SYN expression level and the escape latency of rats was r=-0.916 (P<0.05); the correlation coefficient between SYN expression level and the number of rats entering the wrong entrance holes was r=-0.87 (P<0.05). Conclusion: The impairment of learning and memory function in rats with cerebral ischemia-reperfusion injury may be related to the decrease of SYN expression in the hippocampus, which may result in the damage of synaptic plasticity. |
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