| Objective: To establish and verify the neurogenic bladder (NB) model of rats after complete sacral cord injury. Methods: A total of 12 rats were randomly selected from 42 female SD rats as blank group, and urodynamic test was performed at the 19th day after conventional feeding. The remaining rats were used to establish the NB model of sacral SCI by modified Hassan shaker spinal cord transection, and randomly divided into three experimental groups: groups A, B and C, with 10 rats in each group after modeling. After modeling, the rats in the model group were given standardized nursing care. The bladder function, manual micturition and complications were observed and recorded. At the 19th, 23rd and 28th day after modeling, the rats in the groups A, B and C were tested for urodynamic measurements. Results: Bladder dysfunction in model group was urinary retention within 28 days after modeling; At the 19th day after operation, the vital signs of rats in the model group were stable and the complications were well controlled. As compared with the blank group, pathological changes such as disordered arrangement of detrusor muscle bundles, serious atrophy of muscle fibers and increase of interstitial structure filling were observed in the model group under the light microscope. Urodynamic examination showed that the LPP decreased, the MCC and BC of bladder increased (P<0.01). The results of urodynamics in the model group were compared withthose at the 19th, 23rd and 28th day after modeling, and there were no significant changes in the LPP, MCC and BC of bladder in the three stages, suggesting that dysfunction of urinary retention persisted in rats of model group. Conclusion: Using the improved Hassan shaker method to completely transect the L2-3 spinal cord of SD rats successfully reproduced the NB model of rats, and the quality of the model was stable and rel-iable. |
